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Genetic Insert: 3-color stripe (different sgRNAs)
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32488086
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section. Please note that, in addition, plasmid pJ1996_v2 is needed for the circuit encoded on this plasmid to operate as designed.
Proper citation: RRID:Addgene_140687 Copy
Species: Halothiobacillus neapolitanus
Genetic Insert: CsoS2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4614; Vector Backbone:pET-14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32123388
Proper citation: RRID:Addgene_140841 Copy
Species: Halothiobacillus neapolitanus
Genetic Insert: CsoS2 N-terminal domain (NTD)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4614; Vector Backbone:pET-14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32123388
Proper citation: RRID:Addgene_140842 Copy
Genetic Insert: Control (“C”) lacking sg-1
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32488086
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section. Please note that, in addition, plasmid pJ1996_v2 is needed for the circuit encoded on this plasmid to operate as designed.
Proper citation: RRID:Addgene_140689 Copy
Species: Halothiobacillus neapolitanus
Genetic Insert: CsoS2 C-terminal domain (CTD)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4614; Vector Backbone:pET-14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32123388
Proper citation: RRID:Addgene_140844 Copy
Species: Synthetic
Genetic Insert: sfCFP
Vector Backbone Description: Backbone Size:3231; Vector Backbone:pMB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31361464
Proper citation: RRID:Addgene_140695 Copy
Species: Homo sapiens
Genetic Insert: OsTIR1(F74G)-P2A-mAID-EGFP-NES
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33177522
Proper citation: RRID:Addgene_140730 Copy
Species: Synthetic
Genetic Insert: Cas9m4-KRAB-MeCP2
Vector Backbone Description: Backbone Marker:Andrea Califano; Vector Backbone:PB-TRE-dCas9-KRAB-MeCP2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: This is a lentiviral vector (modified in #122205; originally from Weissman lab #85969) with a Tet-ON 3G dCas9m4-KRAB-MeCP2 (Church lab; #63800). Note: the vector has been modified to use blasticidin selection.
Proper citation: RRID:Addgene_140690 Copy
Genetic Insert: NBαRb-IgG-Fc
Vector Backbone Description: Vector Backbone:pMal-T-Avi-His/BirA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32062022
Proper citation: RRID:Addgene_140699 Copy
Species: Homo sapiens
Genetic Insert: GFP-Pten
Vector Backbone Description: Backbone Size:9248; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29373119
Proper citation: RRID:Addgene_140732 Copy
Species: Homo sapiens
Genetic Insert: GFP-PTEN
Vector Backbone Description: Backbone Size:9248; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29373119
Proper citation: RRID:Addgene_140733 Copy
Species: Homo sapiens
Genetic Insert: BCL2L11
Vector Backbone Description: Backbone Marker:27569; Backbone Size:8804; Vector Backbone:pLenti CMVTRE3G eGFP Neo (w821-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32640215
Proper citation: RRID:Addgene_140747 Copy
Species: Homo sapiens
Genetic Insert: GFP-T2A-PTENW274L
Vector Backbone Description: Backbone Size:9248; Vector Backbone:FUG-PtenW274L; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29373119
Proper citation: RRID:Addgene_140742 Copy
Species: Mus musculus
Genetic Insert: Kir3.2
Vector Backbone Description: Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32372643
Proper citation: RRID:Addgene_140745 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cyclin D3;1
Vector Backbone Description: Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31679162
Proper citation: RRID:Addgene_140750 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p85beta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Cantley lab recommends growth at room temperature.
Proper citation: RRID:Addgene_1408 Copy
Species: Synthetic
Genetic Insert: puromycin-N-acetyltransferase
Vector Backbone Description: Backbone Size:2396; Vector Backbone:pCAGGS; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31597657
Proper citation: RRID:Addgene_140759 Copy
Species: Synthetic
Genetic Insert: Dead-Lapronic
Vector Backbone Description: Backbone Size:5422; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32600029
Proper citation: RRID:Addgene_140753 Copy
Species: Synthetic
Genetic Insert: Mito-Lapronic
Vector Backbone Description: Backbone Size:5671; Vector Backbone:pGW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32600029
Proper citation: RRID:Addgene_140754 Copy
Species: Synthetic
Genetic Insert: Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
Vector Backbone Description: Backbone Size:2846; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32600029
Proper citation: RRID:Addgene_140755 Copy
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