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Species: Synthetic
Genetic Insert: shRenilla
Vector Backbone Description: Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: GFP; shRNA embedded in miRE
Proper citation: RRID:Addgene_135683 Copy
Species: Homo sapiens
Genetic Insert: integrin beta 6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8120056
Proper citation: RRID:Addgene_13580 Copy
Species: E. coli
Genetic Insert: FLIPglu F16A Delta11 Aphrodite
Vector Backbone Description: Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16935985
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
EYFP was exchanged with Aphrodite (accession number DQ525025), which is an codon-diversified version of Venus/EYFP.
Proper citation: RRID:Addgene_13569 Copy
Species: Homo sapiens
Genetic Insert: TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression.
Vector Backbone Description: Backbone Marker:SBI; Backbone Size:7672; Vector Backbone:HR120PA-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31902706
Proper citation: RRID:Addgene_135814 Copy
Species: Homo sapiens
Genetic Insert: integrin alpha 9 / alpha 4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7000; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:11598204
Comments: See article for cloning details.
Proper citation: RRID:Addgene_13587 Copy
Species: Homo sapiens
Genetic Insert: UtrCH
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31264686
Proper citation: RRID:Addgene_135990 Copy
Species: Homo sapiens
Genetic Insert: FMC63-41BB-3z-P2A-EGFP
Vector Backbone Description: Backbone Marker:Heavily modified from LentiCRISPR v1 backbone; Vector Backbone:pSL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32083149
Proper citation: RRID:Addgene_135992 Copy
Species: Homo sapiens
Genetic Insert: FMC63-3z-P2A-EGFP
Vector Backbone Description: Backbone Marker:Heavily modified from LentiCRISPR v1 backbone; Vector Backbone:pSL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32083149
Proper citation: RRID:Addgene_135993 Copy
Genetic Insert: UPF1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32017897
Proper citation: RRID:Addgene_135994 Copy
Species: Homo sapiens
Genetic Insert: G3BP1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32017897
Proper citation: RRID:Addgene_135997 Copy
Species: Homo sapiens
Genetic Insert: G3BP1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32017897
Proper citation: RRID:Addgene_135998 Copy
Vector Backbone Description: Backbone Marker:Eric Kowarz via Addgene; Vector Backbone:pAF111; Vector Types:Mammalian Expression, Luciferase, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32937435
Comments: See bioRxiv preprint: https://doi.org/10.1101/831404 Please see the following article for protocols on how to construct or use this vector: Flies, A. S., Darby, J. M., Murphy, P. R., Pinfold, T. L., Patchett, A. L. & Lennard, P. R. Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins. Bio-protocol under review (2020) https://doi.org/10.21769/BioProtoc.3696.
Proper citation: RRID:Addgene_135920 Copy
Species: Tasmanian devil (Sarcophilus harrisii)
Genetic Insert: TNFRSF9 fused to mCitrine
Vector Backbone Description: Backbone Marker:Andrew Flies Wild Immunology Laboratory; Vector Backbone:pAF137 SalI + NheI digest; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32937435
Comments: See bioRxiv preprint: https://doi.org/10.1101/831404 Please see the following article for protocols on how to construct or use this vector: Flies, A. S., Darby, J. M., Murphy, P. R., Pinfold, T. L., Patchett, A. L. & Lennard, P. R. Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins. Bio-protocol under review (2020) https://doi.org/10.21769/BioProtoc.3696.
Proper citation: RRID:Addgene_135923 Copy
Species: Tasmanian devil (Sarcophilus harrisii)
Genetic Insert: TNFRSF9 fused to mTagBFP
Vector Backbone Description: Backbone Marker:Andrew Flies Wild Immunology Laboratory; Vector Backbone:pAF137 SalI + NheI digest; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32937435
Comments: See bioRxiv preprint: https://doi.org/10.1101/831404 Please see the following article for protocols on how to construct or use this vector: Flies, A. S., Darby, J. M., Murphy, P. R., Pinfold, T. L., Patchett, A. L. & Lennard, P. R. Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins. Bio-protocol under review (2020) https://doi.org/10.21769/BioProtoc.3696.
Proper citation: RRID:Addgene_135924 Copy
Species: Homo sapiens
Genetic Insert: integrin beta 6
Vector Backbone Description: Backbone Marker:Available at Addgene (plasmid #1764); Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Although the SnaBI cloning site was destroyed, there is a BamHI site 5' of the insert and EcoRI and SalI sites 3' of the insert.
Proper citation: RRID:Addgene_13596 Copy
Species: Homo sapiens
Genetic Insert: heterogeneous nuclear ribonucleoprotein D
Vector Backbone Description: Backbone Size:5255; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31665914
Proper citation: RRID:Addgene_135939 Copy
Genetic Insert: UPF1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5 FRT TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32017897
Proper citation: RRID:Addgene_136000 Copy
Genetic Insert: UPF1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5 FRT TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32017897
Proper citation: RRID:Addgene_136002 Copy
Species: Synthetic
Genetic Insert: Prime editor v2 (PE2) N-terminal
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31634902
Proper citation: RRID:Addgene_135955 Copy
Species: Synthetic
Genetic Insert: RNF2_5GtoT pegRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31634902
Proper citation: RRID:Addgene_135957 Copy
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