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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172727 Copy
Species: Synthetic
Genetic Insert: IonicStrengthSensorRD
Vector Backbone Description: Backbone Size:5338; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28853549
Comments: Linker comprises Arginine and Aspartate as positive/negative amino acids.
Proper citation: RRID:Addgene_172931 Copy
Species: Homo sapiens
Genetic Insert: 5HT SENSOR
Vector Backbone Description: Backbone Size:9951; Vector Backbone:pSinRep5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33821000
Proper citation: RRID:Addgene_172882 Copy
Species: Mus musculus
Genetic Insert: Neuralized-like
Vector Backbone Description: Backbone Marker:Conaway Lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 C-FLAG/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18077452
Proper citation: RRID:Addgene_17282 Copy
Vector Backbone Description: Backbone Size:9773; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17292 Copy
Vector Backbone Description: Backbone Size:9594; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17293 Copy
Species: Other
Genetic Insert: mRuby3
Vector Backbone Description: Vector Backbone:pKanCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34100715
Proper citation: RRID:Addgene_172854 Copy
Species: Other
Genetic Insert: DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
Vector Backbone Description: Backbone Size:8484; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34100715
Proper citation: RRID:Addgene_172848 Copy
Species: aquifex aeolicus
Genetic Insert: ultraID
Vector Backbone Description: Backbone Size:8488; Vector Backbone:pSF3; Vector Types:Mammalian Expression, Retroviral, Luciferase, to be used in a tet-on or tet-off compatible cell line; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35788163
Proper citation: RRID:Addgene_172878 Copy
Species: Synthetic
Genetic Insert: Ace2N-4AA-mNeon A122D
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37015225
Comments: Please visit https://doi.org/10.1101/2021.11.09.467909 for bioRxiv preprint.
Proper citation: RRID:Addgene_172911 Copy
Species: Synthetic
Genetic Insert: jGCaMP7s-T2A-tdTomato
Vector Backbone Description: Backbone Marker:Gerald Rubin; Vector Backbone:derived from pJFRC7; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_172916 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Marker:Olivier Destree; Backbone Size:5500; Vector Backbone:pGlomyc (based on pCDNA3.1); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.22.449461v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172870 Copy
Vector Backbone Description: Backbone Size:9014; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: Please note that there are several mismatches in sequence between depositor's sequence and Addgene's quality control sequence for this plasmid. These mismatches are in backbone regions and should not affect function.
Proper citation: RRID:Addgene_17297 Copy
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid 26106); Vector Backbone:pNH-TrxT (but TrxT removed); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34786571
Comments: pNH-TrxT that contains the sequence of Nanoluciferase in place of TrxT before the TEV cleavage site. Please visit https://www.biorxiv.org/content/10.1101/2021.05.31.445082v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_173075 Copy
Species: Prevotella pectinovora
Genetic Insert: pspCas13b
Vector Backbone Description: Backbone Size:9202; Vector Backbone:EF1a-lenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34257311
Proper citation: RRID:Addgene_173029 Copy
Species: Rattus norvegicus
Genetic Insert: PV-NES-GFP
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pCMV-Myc-Cyto-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11971908
Proper citation: RRID:Addgene_17301 Copy
Species: Feline Immunodeficiency Virus
Genetic Insert: Gag-Pol and Rev
Vector Backbone Description: Backbone Size:12905; Vector Backbone:modified pUC119; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
Proper citation: RRID:Addgene_1732 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7499314
Proper citation: RRID:Addgene_17327 Copy
Species: Streptomyces avidinii
Genetic Insert: streptavidin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET-3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11716692
Comments: A PCR-based site-directed mutagenesis method was used to modify the coding sequence of pTSA-13. A DNA sequence encoding six amino acid residues with a translation termination codon, GGT GGT TCT GGT TGC CCG TAG (Gly-Gly-Ser-Gly-Cys-Pro-Stop), was fused to the codon for Val-133 (GTG). The entire coding sequence was cloned between the NdeI and BamHI sites of the plasmid pET-3a under the control of the promoter of bacteriophage T7.
Proper citation: RRID:Addgene_17329 Copy
Species: Homo sapiens
Genetic Insert: Calcineurin Like EF-Hand Protein 1
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30846317
Proper citation: RRID:Addgene_173166 Copy
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