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Species: Homo sapiens
Genetic Insert: Anillin (AHD+PH)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18158243
Proper citation: RRID:Addgene_68026 Copy
Species: Synechococcus elongatus
Genetic Insert: KaiA
Vector Backbone Description: Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23071342
Comments: Ulp1 cutting site introduced before KaiA. SUMO-KaiA cloned between NdeI and Hind III. No enterokinase cutting site. Use BL21DE3 and Ni column for protein purification.
Proper citation: RRID:Addgene_68049 Copy
Species: Herpesvirus saimiri 2
Genetic Insert: HSUR4
Vector Backbone Description: Backbone Marker:Integrated DNA Technologies; Backbone Size:1962; Vector Backbone:pIDTSmart-Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24713319
Proper citation: RRID:Addgene_68052 Copy
Species: Synechococcus elongatus
Genetic Insert: KaiC
Vector Backbone Description: Vector Backbone:pET41a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23071342
Comments: PreScission (GE Healthcare) Protease cutting site is introduced before KaiC. KaiC is cloned between NcoI and HiNdII. Use BL21DE3 and GST column for protein purification. Use PreScission Protease to cut GST tag off.
Proper citation: RRID:Addgene_68051 Copy
Species: Synechococcus elongatus
Genetic Insert: KaiB
Vector Backbone Description: Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23071342
Comments: Ulp1 cutting site introduced before KaiB. Sumo-KaiB cloned between NdeI and HindIII. No enterokinase cutting site. Use BL21DE3 and Ni column for protein purification.
Proper citation: RRID:Addgene_68050 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26158227
Comments: Derived from Venus. Human codon optimized and contains the sfGFP and cysteine to serine mutations.
Excitation/Emission 514/526
Brightness 44 (product of extinction coefficient and quantum yield and divided by 1,000).
Proper citation: RRID:Addgene_68067 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26158227
Comments: Derived from Cerulean3.
Excitation/Emission 434/474
Brightness 36 (product of extinction coefficient and quantum yield and divided by 1,000).
Proper citation: RRID:Addgene_68066 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26158227
Comments: Derived from EBFP2. Changed C48S, C70S and V163A
Excitation/Emission 385/448
Brightness 17 (product of extinction coefficient and quantum yield and divided by 1,000).
Proper citation: RRID:Addgene_68063 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26158227
Comments: Derived from sfGFP. (cysteines removed)
Excitation/Emission 486/510
Brightness 50 (product of extinction coefficient and quantum yield and divided by 1,000).
Proper citation: RRID:Addgene_68069 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26158227
Proper citation: RRID:Addgene_68068 Copy
Species: unknown
Genetic Insert: GalT signal anchor transmembrane domain
Vector Backbone Description: Backbone Marker:Snapp lab, Addgene Plasmid 68063; Vector Backbone:oxBFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26158227
Proper citation: RRID:Addgene_68073 Copy
Species: E. coli
Genetic Insert: aac3(IV)
Vector Backbone Description: Vector Backbone:pSET152; Vector Types:Streptomyces integrating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12923110
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_68113 Copy
Species: Other
Genetic Insert: Luc / Renilla / P19
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3alpha1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68218 Copy
Species: Other
Genetic Insert: Luc
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1alpha1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68217 Copy
Species: Other
Genetic Insert: Luc
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1alpha1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68216 Copy
Species: Other
Genetic Insert: Renilla
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1alpha1; Vector Types:Plant Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68215 Copy
Species: Other
Genetic Insert: P19 silencing suppressor
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Vector Backbone:pDGB3alpha2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68214 Copy
Species: Other
Genetic Insert: Hyg
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1alpha1R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68213 Copy
Species: Other
Genetic Insert: NptII
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1alpha1R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68212 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Backbone Marker:self-made; derived from pGreenII generated at the JIC; Vector Backbone:pDGB1alpha2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23669743
Comments: insert can be released with BsmBI
Proper citation: RRID:Addgene_68220 Copy
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