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Genetic Insert: 2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37696818
Proper citation: RRID:Addgene_209555 Copy
Genetic Insert: SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37696818
Proper citation: RRID:Addgene_209556 Copy
Species: Zea mays
Genetic Insert: ZmUbi1 promoter
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:pMC-1-ccdB-6; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36896468
Comments: Please visit https://doi.org/10.1101/2022.10.15.511792 for bioRxiv preprint.
Proper citation: RRID:Addgene_197714 Copy
Species: (TurboID) BirA from E. coli. cTP is RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (AT1G67090).
Genetic Insert: PM-3XHA-TurbOID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171).
Proper citation: RRID:Addgene_209403 Copy
Species: (TurboID) BirA from E. coli. mTP is from GAMMA SUBUNIT OF MT ATP SYNTHASE (AT2G33040).
Genetic Insert: mTP-3XHA-TurboID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209404 Copy
Species: (TurboID) BirA from E. coli. PTS1 is from AUTOPHAGY-RELATED PROTEIN 1A (AT3G61960). PTS1 sequence is SNLQHRRSHL.
Genetic Insert: 3XHA-TurboID-PTS1
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209406 Copy
Species: (TurboID) BirA from E. coli; NLS is from simian virus 40 (SV40) large T antigen .
Genetic Insert: 3XHA-TurboID-3XNLS
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171).
Proper citation: RRID:Addgene_209400 Copy
Species: Geobacillus Stearothermophilus
Genetic Insert: GstTnpB3
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37758954
Proper citation: RRID:Addgene_210872 Copy
Species: Geobacillus Stearothermophilus
Genetic Insert: GstTnpB2
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37758954
Proper citation: RRID:Addgene_210871 Copy
Species: Panicum virgatum
Genetic Insert: PvUbi1 promoter
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:pMC-1-ccdB-6; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36896468
Comments: Please visit https://doi.org/10.1101/2022.10.15.511792 for bioRxiv preprint.
Proper citation: RRID:Addgene_197705 Copy
Species: (TurboID) BirA from E. coli. PM is fom LTI6B (AT3G05890).
Genetic Insert: PM-3XHA-TurbOID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171).
Proper citation: RRID:Addgene_209395 Copy
Species: (TurboID) BirA from E. coli. mTP is from GAMMA SUBUNIT OF MT ATP SYNTHASE (AT2G33040).
Genetic Insert: mTP-3XHA-TurboID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209396 Copy
Species: (TurboID) BirA from E. coli. GTS is from ALPHA-MANNOSIDASE 3 (AT1G30000) .
Genetic Insert: GTS-3XHA-TurboID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209397 Copy
Species: (TurboID) BirA from E. coli
Genetic Insert: 3XHA-TurboID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209391 Copy
Species: (TurboID) BirA from E. coli;NES is from HEAT STABLE INHIBITOR (PKI) .
Genetic Insert: NES-3XHA-TurboID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209393 Copy
Species: (TurboID) BirA from E. coli
Genetic Insert: 3XHA-TurboID
Vector Backbone Description: Backbone Marker:Parniske lab; Vector Backbone:LII alpha F1-2; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38294100
Comments: 3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
Proper citation: RRID:Addgene_209399 Copy
Species: Triticum aestivum
Genetic Insert: Sr27
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_201307 Copy
Species: Triticum aestivum
Genetic Insert: D16: Promoter of TraesCS5D02G320700
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_201306 Copy
Species: Synthetic
Genetic Insert: PgpdA:yfp:TtrpC::Ppyr4:pyr4:Tpyr4
Vector Backbone Description: Vector Backbone:pDGB3Ω1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_209129 Copy
Species: Synthetic
Genetic Insert: PafpA:afpA:TafpA::Ppyr4:pyr4:Tpyr4
Vector Backbone Description: Vector Backbone:pDGB3Ω1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: We find a nucleotide discrepancy in PafpA at position 555 that changes a C to an A.
Proper citation: RRID:Addgene_209130 Copy
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