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Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182171 Copy
Species: Synthetic
Genetic Insert: mVenusNB-MrkHR113A
Vector Backbone Description: Backbone Marker:J. H. Davis, A. J. Rubin, R. T. Sauer, Design, construction and characterization of a set of insulated bacterial promoters.; Backbone Size:3200; Vector Backbone:pEB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35131853
Comments: We obtained the codon optimized MrkH sequence from Maria Schumacher, as described in her publication PMID: 27551088
Proper citation: RRID:Addgene_182292 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182173 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-GLuc
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182213 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Vector Backbone:pBluescript II KS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143616
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.11.18.469106v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_182179 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-Akaluc
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, TEM imaging; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182212 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18222 Copy
Species: Homo sapiens
Genetic Insert: NFIA
Vector Backbone Description: Backbone Size:13000; Vector Backbone:pUCM; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182306 Copy
Species: Homo sapiens
Genetic Insert: MYOD1-shOct4
Vector Backbone Description: Backbone Size:13225; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182309 Copy
Species: Brevibacillus sp. SYP-B805
Genetic Insert: BrCas12b
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35290826
Proper citation: RRID:Addgene_182276 Copy
Species: Homo sapiens
Genetic Insert: hNGN2-P2A-EMX1
Vector Backbone Description: Backbone Size:13225; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182312 Copy
Species: Mus musculus
Genetic Insert: pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
Vector Backbone Description: Backbone Size:3100; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35649726
Proper citation: RRID:Addgene_182246 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7344; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35143476
Proper citation: RRID:Addgene_182254 Copy
Genetic Insert: neo/FLAG TAP
Vector Backbone Description: Backbone Size:1801; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1822 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18219 Copy
Species: Synthetic
Genetic Insert: T2A-tdTomato-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182340 Copy
Species: Synthetic
Genetic Insert: T2A-TagRFP-T-linker-dre-PBD*-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182342 Copy
Species: Other
Genetic Insert: KSHV Kaposincluster_RevCompl_KapATGdel_SYN_rev
Vector Backbone Description: Vector Backbone:pcDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182624 Copy
Species: Other
Genetic Insert: antiPAN_Rev
Vector Backbone Description: Vector Backbone:pDEST47; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182628 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Primers for LIC cloning: Upstream: add TATTTTCAATCCTACGTA to the 5’ end of gene of interest. Downstream: add CCCTCAATATTATACGGG to the 3’ end of GOI. Detailed cloning method available in the paper Bruni R, Kloss B. High-throughput cloning and expression of integral membrane proteins in Escherichia coli. Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34.
Proper citation: RRID:Addgene_182506 Copy
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