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Species: Danio rerio
Genetic Insert: zebrafish ttn.1_C3 sgRNA for in vitro transcription
Vector Backbone Description: Vector Backbone:pT7gRNA-ccdb (Addgene 140864); Vector Types:In vitro transcription of sgRNAs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140866 Copy
Species: Homo sapiens
Genetic Insert: GGamma9-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5432; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32367019
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_140991 Copy
Species: Synthetic
Genetic Insert: TetR-sfGFP expressed with T3 promoter
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30487584
Proper citation: RRID:Addgene_140871 Copy
Species: phage T3
Genetic Insert: T3 RNA polymerase
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30487584
Proper citation: RRID:Addgene_140872 Copy
Species: Discosoma
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pMV361-strep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:32829077
Comments: tdTomato: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Shaner Nc, Campbell Re, Steinbach Pa, Giepmans Bng, Palmer Ae, Tsien Ry
(2004). Nature Biotechnology, 22(12) , 1567-1572. doi: 10.1038/nbt1037.
Proper citation: RRID:Addgene_140994 Copy
Species: Mus musculus
Genetic Insert: PI3K regulatory subunit p55alpha
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: does not express, only to subclone into other plasmid, contains internal BglII site
Proper citation: RRID:Addgene_1409 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR-221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140874 Copy
Species: Danio rerio
Genetic Insert: Rab8b
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140881 Copy
Species: Danio rerio
Genetic Insert: Rab33bb
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140882 Copy
Species: simian virus
Genetic Insert: Large T antigen
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12781366
Proper citation: RRID:Addgene_14088 Copy
Species: Homo sapiens
Genetic Insert: Ago Chi-c
Vector Backbone Description: Backbone Size:5409; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16061186
Proper citation: RRID:Addgene_14086 Copy
Species: Mus musculus
Genetic Insert: p19 ARF shRNA -2
Vector Backbone Description: Backbone Marker:Available from Addgene (#11795); Backbone Size:8000; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12853964
Comments: p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.
Proper citation: RRID:Addgene_14091 Copy
Species: Danio rerio
Genetic Insert: Rab38a
Vector Backbone Description: Vector Backbone:pCSDEST2 (Addgene 22424); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_140887 Copy
Species: Danio rerio
Genetic Insert: CiDr-VSP L223F/C302S-mCherry
Vector Backbone Description: Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32167537
Proper citation: RRID:Addgene_140893 Copy
Species: Homo sapiens
Genetic Insert: Human RhoC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3519; Vector Backbone:PCR-BluntII-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10952316
Proper citation: RRID:Addgene_14099 Copy
Species: Mus musculus
Genetic Insert: goosecoid
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17142318
Proper citation: RRID:Addgene_14096 Copy
Species: Mus musculus
Genetic Insert: goosecoid
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pWZL Blast; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17142318
Proper citation: RRID:Addgene_14095 Copy
Vector Backbone Description: Backbone Marker:pUA66 was described in Zaslaver et al. (2006) Nat. Methods 3: 623-628.; Backbone Size:4849; Vector Backbone:Derived from pUA66; Vector Types:Bacterial Expression, Vector for the I-Block assay designed to study DNA-protein interaction in E. coli.; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31980824
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_141079 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:pCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141112 Copy
Species: Homo sapiens
Genetic Insert: HMETTL14
Vector Backbone Description: Vector Backbone:PCDH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141113 Copy
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