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Species: Homo sapiens
Genetic Insert: EIF3B 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:Modified psCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32017897
Proper citation: RRID:Addgene_136034 Copy
Species: Homo sapiens
Genetic Insert: UPF1
Vector Backbone Description: Vector Backbone:PLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32017897
Proper citation: RRID:Addgene_136036 Copy
Species: Homo sapiens
Genetic Insert: Presenilin-associated rhomboid-like
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17116872
Proper citation: RRID:Addgene_13617 Copy
Species: Homo sapiens
Genetic Insert: GST-p18
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30181260
Proper citation: RRID:Addgene_136144 Copy
Species: Synthetic
Genetic Insert: rtTA-Advanced::P2A::EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5070; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit that contains the regulator reverse tetracycline Trans Activator (rtTA) and the reporter EGFP, which is controlled by a tight TRE promoter that rtTA binds to activate its own expression in a positive feedback loop. The backbone consists of the Flp-In expression vector (pcDNA5/FRT). The synthetic gene circuit is also called mPF-GFP.
Proper citation: RRID:Addgene_128254 Copy
Species: Synthetic
Genetic Insert: Beta Globin Intron::hTetR::P2A::EGFP
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:7505; Vector Backbone:pDN-D2irTNG5kwh; Vector Types:Mammalian Expression, Synthetic Biology, Flp-In expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31235692
Comments: The plasmid encodes a mammalian synthetic gene circuit with the humanized Tetracycline Repressor (hTetR) regulator separated from EGFP by a P2A motif, which is controlled by a CMV-derived promoter containing 2 tetO2 sites. With hTetR binding to the promoter, negative feedback lowers gene expression noise while linearizing the mean dose response. The circuit is also called mNF-GFP and is within a Flp-In expression vector.
Proper citation: RRID:Addgene_128253 Copy
Species: Homo sapiens
Genetic Insert: TEX264 LIR4A
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128259 Copy
Species: Homo sapiens
Genetic Insert: TEX264
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128258 Copy
Species: Other
Genetic Insert: ssRFPGFPKDEL
Vector Backbone Description: Backbone Size:7710; Vector Backbone:pCW57-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128257 Copy
Species: Synthetic
Genetic Insert: ZmUBIp::ScCas9 (wheat codon optimized):NOSt
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32326901
Proper citation: RRID:Addgene_128192 Copy
Species: Rhodosporidium toruloides
Genetic Insert: gRNA cloning cassette
Vector Backbone Description: Backbone Size:6303; Vector Backbone:pZPK; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128178 Copy
Species: Rhodosporidium toruloides
Genetic Insert: SpCas9-NLS3
Vector Backbone Description: Backbone Marker:Zhao lab; Backbone Size:13658; Vector Backbone:pCAMBRIA; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31038202
Proper citation: RRID:Addgene_128177 Copy
Species: Homo sapiens
Genetic Insert: shcGAS Mmu
Vector Backbone Description: Backbone Size:13320; Vector Backbone:pTRIPZ RFP+; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28598415
Proper citation: RRID:Addgene_128175 Copy
Species: Homo sapiens
Genetic Insert: BMI1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29891515
Comments: The insert for BMI1 contains an S572P compared to the reference NP_001190991.1 that does not affect function.
Proper citation: RRID:Addgene_128328 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:Addgene # 55641; Backbone Size:6323; Vector Backbone:pAAV-Ef1a-fDIO; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35984878
Proper citation: RRID:Addgene_128315 Copy
Species: Synthetic
Genetic Insert: TetR-LOV-RRRG
Vector Backbone Description: Vector Backbone:pCB-D2irTN5kwh; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31269201
Comments: Additional relevant information can be found from this publciation: Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471
Proper citation: RRID:Addgene_128271 Copy
Species: Homo sapiens
Genetic Insert: SEC62
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6412; Vector Backbone:pMRX-INU-FLAG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31006538
Proper citation: RRID:Addgene_128263 Copy
Species: Homo sapiens
Genetic Insert: Human Nucleic Acid Binding Protein 2 (hNABP2)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550690
Comments: Y85C mutation does not affect plasmid function. Point mutation is located in the middle of the OB fold, and no particular function is reported/known for this residue.
Proper citation: RRID:Addgene_128307 Copy
Species: Homo sapiens
Genetic Insert: Human nucleic acid-binding protein 1 (hNABP1)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26550690
Proper citation: RRID:Addgene_128306 Copy
Species: Caenorhabditis elegans
Genetic Insert: vab-1
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pSL1190; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9506518
Proper citation: RRID:Addgene_128414 Copy
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