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Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74811 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74818 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74807 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74806 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74796 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74797 Copy
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pCM66; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Similar backbone to pAWP78 (AddGene plasmid 61263)
Proper citation: RRID:Addgene_74738 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74859 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74856 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Comments: Please note that pUC primers are not recommended for sequencing due to the inverted repeat of the Tnos sequence which forms a secondary structure and prevents sequencing reactions. If you need to sequence or PCR the Tnos region, digestion by AscI helps separates two Tnos sequences and prevents the secondary structure.
Proper citation: RRID:Addgene_74843 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74848 Copy
Vector Backbone Description: Backbone Size:9957; Vector Backbone:pGAMA-empty; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26947063
Comments: Gateway destination lentiviral vector. Gene of interest introduced by Gateway reaction (with stop codon removed) will be driven by EF1a promoter, with a V5 C-terminal tag followed by T2A mCherry. Cells will turn red fluorescent.
Proper citation: RRID:Addgene_74755 Copy
Species: Synthetic
Genetic Insert: KillerOrange
Vector Backbone Description: Backbone Marker:QIAGEN; Backbone Size:3500; Vector Backbone:pQE-30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26679300
Proper citation: RRID:Addgene_74748 Copy
Species: Homo sapiens
Genetic Insert: COMMD3
Vector Backbone Description: Backbone Size:5350; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15799966
Proper citation: RRID:Addgene_74895 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74884 Copy
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Proper citation: RRID:Addgene_74883 Copy
Species: Homo sapiens
Genetic Insert: Titin I27 x 9
Vector Backbone Description: Backbone Size:5441; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27276010
Comments: Each Titin I27 domain is separated by a 4 amino acid linker - two aa being "RS" and the other two corresponding to the specific restriction site
Proper citation: RRID:Addgene_74888 Copy
Species: Homo sapiens
Genetic Insert: NSF
Vector Backbone Description: Backbone Marker:LifeTechnologies; Vector Backbone:pcDNA3-NTAP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26758690
Proper citation: RRID:Addgene_74924 Copy
Species: Phaeodactylum tricornutum
Genetic Insert: LHCF2 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2904; Vector Backbone:pBlueScript KS(+); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27108533
Comments: The diaCas9 module was synthesized by GeneArt® Services Thermo Fisher Scientific Inc.
The sgRNA module was synthesized by Eurofins Genomic Services Ltd.
Proper citation: RRID:Addgene_74923 Copy
Genetic Insert: 24xGCN4_v4-BFP-24xPP7
Vector Backbone Description: Vector Backbone:pcDNA4TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27153498
Proper citation: RRID:Addgene_74929 Copy
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