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Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:custom; Vector Types:Bacterial Expression, CRISPR, Fungal expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26177455
Proper citation: RRID:Addgene_87843 Copy
Species: Synthetic
Genetic Insert: sgGFP1, sgCas9
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:2600; Vector Backbone:pENTR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28930690
Proper citation: RRID:Addgene_87908 Copy
Species: Synthetic
Genetic Insert: sgHTT1, shGFP2, sgCas9, cherry
Vector Backbone Description: Backbone Size:7600; Vector Backbone:HIV-1 SIN lentiviral transfer vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28930690
Proper citation: RRID:Addgene_87919 Copy
Species: Synthetic
Genetic Insert: sgHTT1, sgGFP2, shCas9
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:2600; Vector Backbone:pENTR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28930690
Proper citation: RRID:Addgene_87917 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRC2 3xFLAG; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28174228
Proper citation: RRID:Addgene_87778 Copy
Species: Synthetic
Genetic Insert: {T_AtHsp18.2}
Vector Backbone Description: Vector Backbone:pBca9145 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27782150
Proper citation: RRID:Addgene_87968 Copy
Species: Synthetic
Genetic Insert: pZ-YFP
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pNH603; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27930885
Proper citation: RRID:Addgene_87946 Copy
Species: Synthetic
Genetic Insert: GGG-ELP(40nm)-Cys
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5219; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28591514
Comments: The underlying cloning procedure of the employed ELP genes is described in this article:
Ott, W.; Nicolaus, T.; Gaub, H. E.; Nash, M. A. Sequence-Independent Cloning and Post-Translational Modification of Repetitive Protein Polymers through Sortase and Sfp-Mediated Enzymatic Ligation. Biomacromolecules 2016, 17, 1330−1338.
Proper citation: RRID:Addgene_91571 Copy
Species: Synthetic
Genetic Insert: mEGFP-P2A-paAIP2
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28318784
Proper citation: RRID:Addgene_91718 Copy
Species: Synthetic
Genetic Insert: FKBP_F36V-2xHA-P2A-Puro
Vector Backbone Description: Backbone Marker:Takashi Yamamoto lab; Backbone Size:4579; Vector Backbone:pCRIS-PITChv2 (addgene #63672); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581585
Comments: See:
Sakuma, T., Nakade, S., Sakane, Y., Suzuki, K.-I. & Yamamoto, T. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc 11, 118–133 (2016).
for the original backbone and general cloning protocol.
Proper citation: RRID:Addgene_91796 Copy
Species: Synthetic
Genetic Insert: FKBP_F36V-2xHA-P2A-BSD
Vector Backbone Description: Backbone Marker:Takashi Yamamoto lab; Backbone Size:4579; Vector Backbone:pCRIS-PITChv2 (addgene #63672); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581585
Comments: See:
Sakuma, T., Nakade, S., Sakane, Y., Suzuki, K.-I. & Yamamoto, T. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc 11, 118–133 (2016).
for the original backbone and general cloning protocol.
Proper citation: RRID:Addgene_91795 Copy
Species: Synthetic
Genetic Insert: Puro-P2A-2xHA-FKBP_F36V
Vector Backbone Description: Backbone Marker:Takashi Yamamoto lab; Backbone Size:4579; Vector Backbone:pCRIS-PITChv2 (addgene #63672); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29581585
Comments: See:
Sakuma, T., Nakade, S., Sakane, Y., Suzuki, K.-I. & Yamamoto, T. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc 11, 118–133 (2016).
for the original backbone and general cloning protocol.
Proper citation: RRID:Addgene_91793 Copy
Species: Synthetic
Genetic Insert: LoxP-SEC-LoxP
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91829 Copy
Species: Synthetic
Genetic Insert: mScarlet-I-C1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91826 Copy
Species: Synthetic
Genetic Insert: HaloTag-C1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91827 Copy
Species: Synthetic
Genetic Insert: Lox511I-SEC-Lox511I
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32550377
Proper citation: RRID:Addgene_91831 Copy
Species: Synthetic
Genetic Insert: LwCas13a Guide site
Vector Backbone Description: Backbone Size:2850; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91906 Copy
Species: Synthetic
Genetic Insert: LOV2
Vector Backbone Description: Vector Backbone:pBABE; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27980211
Proper citation: RRID:Addgene_91873 Copy
Species: Synthetic
Genetic Insert: LwCas13a Guide site
Vector Backbone Description: Backbone Size:2708; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91907 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:F. Zhang Lab (Addgene #60229); Backbone Size:6394; Vector Backbone:AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28888955
Proper citation: RRID:Addgene_91948 Copy
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