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Species: Homo sapiens
Genetic Insert: GCN5
Vector Backbone Description: Backbone Size:25000; Vector Backbone:pAdEasy; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: Flag-GCN5 was cloned into the pAdTrack shuttle vector and adenovirus constructs were created by recombination of the shuttle vector and pAdEasy vector by electroporation into BJ5183-AD-1 bacteria (Stratagene).
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_14106 Copy
Species: Homo sapiens
Genetic Insert: CMYB
Vector Backbone Description: Vector Backbone:pmiR-report; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29290617
Proper citation: RRID:Addgene_141114 Copy
Species: Homo sapiens
Genetic Insert: Ago2
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15800629
Proper citation: RRID:Addgene_14104 Copy
Species: Homo sapiens
Genetic Insert: Ago2
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15800629
Proper citation: RRID:Addgene_14102 Copy
Species: Homo sapiens
Genetic Insert: Ago2
Vector Backbone Description: Backbone Size:5368; Vector Backbone:pDESTFLAGHApuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15800629
Proper citation: RRID:Addgene_14103 Copy
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG FlpE to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Please visit https://www.biorxiv.org/content/10.1101/571323v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141111 Copy
Genetic Insert: HyPer7
Vector Backbone Description: Backbone Size:3425; Vector Backbone:pQE-30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32130885
Proper citation: RRID:Addgene_141071 Copy
Species: Synthetic
Genetic Insert: RexYFP
Vector Backbone Description: Backbone Size:3425; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24286672
Proper citation: RRID:Addgene_141070 Copy
Species: Homo sapiens
Genetic Insert: Antares-T2A-puromycin-EMCV IRES-SEPLuc
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4606; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Comments: Deletion in the luciferase does not affect the BRET efficiency of Antares
Proper citation: RRID:Addgene_141086 Copy
Species: Homo sapiens
Genetic Insert: Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4606; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Proper citation: RRID:Addgene_141089 Copy
Species: Homo sapiens
Genetic Insert: SEPLuc-E2A-cyOFP1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Proper citation: RRID:Addgene_141084 Copy
Species: Homo sapiens
Genetic Insert: SEPLuc-EMCV IRES-Antares
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:5386; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32457886
Proper citation: RRID:Addgene_141085 Copy
Genetic Insert: zCas9(H840A)-OsM-MLV-RT
Vector Backbone Description: Vector Backbone:pYPQ165; Vector Types:Plant Expression, CRISPR, Gateway compatible zCas9(H840A)-OsM-MLV-RT; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32222487
Comments: Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157.
Proper citation: RRID:Addgene_141080 Copy
Species: Synthetic
Genetic Insert: TKBsd
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141009 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141013 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a T to C nucleotide mutation in attP1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141014 Copy
Species: Corynebacterium diphtheriae
Genetic Insert: DTA
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141011 Copy
Species: S. pyogenes
Genetic Insert: Cas9 and tracrRNA
Vector Backbone Description: Vector Backbone:colE1, pAM beta; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Defining Citation: PMID:30156038
Proper citation: RRID:Addgene_141095 Copy
Vector Backbone Description: Vector Backbone:colE1, pAM beta; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30156038
Proper citation: RRID:Addgene_141096 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:4416; Vector Backbone:pPEPZ; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33120116
Proper citation: RRID:Addgene_141090 Copy
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