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Species: Homo sapiens
Genetic Insert: CLK1
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:7756; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34346309
Proper citation: RRID:Addgene_174088 Copy
Species: Homo sapiens
Genetic Insert: SRRM2
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:7756; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34346309
Proper citation: RRID:Addgene_174089 Copy
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:Jacks Lab; Backbone Size:9200; Vector Backbone:Puro.Cre; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18308936
Comments: Puro.Cre was initially generated by amplification of PuroR from MSCV.Puro (Clontech) with PuroF (5’-CGCTGCGCCGAATTCATGACCGAGTACAAGCCCACG-3’) and PuroR (5’-TAAGCGGCCGCTCGAGTCAGGCACCGGGCTTGCGGGTC-3’), digestion with EcoRI and NotI and cloning into UbC.Luciferase.PGK.Cre to generate Puro.Cre.
For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
Proper citation: RRID:Addgene_17408 Copy
Species: Homo sapiens
Genetic Insert: CCND1
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:VectorBuilder pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174155 Copy
Species: Homo sapiens
Genetic Insert: AMBRA1
Vector Backbone Description: Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174157 Copy
Species: Homo sapiens
Genetic Insert: Control sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174149 Copy
Species: Aequorea victoria
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pSin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33986151
Proper citation: RRID:Addgene_174307 Copy
Species: Synthetic
Genetic Insert: dCas9, 10xSunTag, tagBFP
Vector Backbone Description: Backbone Size:5706; Vector Backbone:AmpR-Ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31941101
Comments: Shorter version of the original plasmid, in which a 4.5-kb fragment in the vector backbone was deleted
Proper citation: RRID:Addgene_174141 Copy
Species: Homo sapiens
Genetic Insert: Control sgRNA
Vector Backbone Description: Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854239
Proper citation: RRID:Addgene_174148 Copy
Vector Backbone Description: Backbone Size:8020; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270926
Proper citation: RRID:Addgene_174175 Copy
Vector Backbone Description: Backbone Size:10140; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: Please note that there are several sequence discrepancies between Addgene's sequence and depositor's sequence. These mismatches are in backbone regions and should not affect function.
Proper citation: RRID:Addgene_17431 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Size:7900; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270926
Proper citation: RRID:Addgene_174170 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Size:7900; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34270926
Proper citation: RRID:Addgene_174168 Copy
Species: synthetic
Genetic Insert: Cas9-BFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34697476
Comments: Please visit https://www.biorxiv.org/content/10.1101/775080v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_174162 Copy
Species: Mus musculus
Genetic Insert: MusCK Library (M_a)
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 52961); Vector Backbone:lentiCRISPR-v2-puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34582788
Proper citation: RRID:Addgene_174196 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR4; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17414 Copy
Species: Other
Genetic Insert: mCherry with mitchondria-targeting sequence
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFX; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174188 Copy
Species: Other
Genetic Insert: AcGFP with mitochondria-targeting sequence
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31735740
Proper citation: RRID:Addgene_174180 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3802; Vector Backbone:pENTR1A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_17428 Copy
Species: Candida auris
Genetic Insert: C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
Vector Backbone Description: Backbone Size:2236; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174432 Copy
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