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Species: Candida auris
Genetic Insert: HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174433 Copy
Species: Synthetic
Genetic Insert: Candida optimized monomeric GFP
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174434 Copy
Species: Drosophila melanogaster
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531964
Proper citation: RRID:Addgene_174421 Copy
Species: Homo sapiens
Genetic Insert: TRAP220
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pWZL Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18039840
Proper citation: RRID:Addgene_17433 Copy
Species: Mus musculus
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pCS2+ NLSMT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18003914
Comments: Full length mouse JMJD3 with a C-terminal FLAG was PCR-amplified from MGC clone BC075632 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
Proper citation: RRID:Addgene_17440 Copy
Vector Backbone Description: Backbone Marker:Philippa Marrack Lab; Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16873067
Comments: Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.
Proper citation: RRID:Addgene_17442 Copy
Species: Homo sapiens
Genetic Insert: FOXP3
Vector Backbone Description: Backbone Size:6063; Vector Backbone:MSCV-IRES-Thy1.1 DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16873067
Proper citation: RRID:Addgene_17443 Copy
Species: Other
Genetic Insert: protein A - hia5
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35396487
Comments: The depositing laboratory recommends Addgene Plasmid 222303: prhaBAD-pA-Hia5, an improved version of this plasmid.
Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye
Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383
Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646
Proper citation: RRID:Addgene_174372 Copy
Genetic Insert: TEV Protease
Vector Backbone Description: Vector Backbone:pB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37053554
Proper citation: RRID:Addgene_174377 Copy
Genetic Insert: FLPo recombinase
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38212587
Proper citation: RRID:Addgene_174378 Copy
Species: Candida auris
Genetic Insert: NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174405 Copy
Species: Candida auris
Genetic Insert: C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
Vector Backbone Description: Backbone Size:2242; Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34730409
Proper citation: RRID:Addgene_174409 Copy
Species: Streptomyces tendae
Genetic Insert: nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Vector Backbone Description: Backbone Size:3516; Vector Backbone:pCm No carrier; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35101207
Proper citation: RRID:Addgene_174361 Copy
Species: Salmonella enterica ser. Typhimurium
Genetic Insert: sarA
Vector Backbone Description: Vector Backbone:pWSK129; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31901521
Proper citation: RRID:Addgene_174401 Copy
Species: Homo sapiens
Genetic Insert: gRNA against TRAC
Vector Backbone Description: Vector Backbone:pXPR_001 (lentiCRISPRv1, Addgene #49535); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_164993 Copy
Species: Homo sapiens
Genetic Insert: TRAV
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33483338
Proper citation: RRID:Addgene_164999 Copy
Species: Homo sapiens
Genetic Insert: Pig3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4500; Vector Backbone:pBK-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9305847
Proper citation: RRID:Addgene_16496 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9660945
Comments: Four copies of the Smad binding site (GTCTAGAC) are cloned into pBV-Luc.
Proper citation: RRID:Addgene_16495 Copy
Species: Homo sapiens
Genetic Insert: Rheb
Vector Backbone Description: Backbone Marker:Available at Addgene (#19319); Backbone Size:7400; Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157014
Proper citation: RRID:Addgene_165031 Copy
Species: Homo sapiens
Genetic Insert: BACE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22553349
Proper citation: RRID:Addgene_165032 Copy
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