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Species: Synthetic
Genetic Insert: Ancestral AAV Capsid Anc80L65
Vector Backbone Description: Backbone Size:5238; Vector Backbone:synthetic; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26235624
Comments: An improved version of this plasmid from the Vandenberghe Lab is available. Please see pAnc80L65AAP (Addgene plasmid# 92307).
This plasmid, pAnc80L65 (#68837), requires cotransfection with a plasmid expressing AAP. For efficient viral vector production without cotransfecting AAP, use pAnc80L65AAP (Addgene plasmid# 92307)
Proper citation: RRID:Addgene_68837 Copy
Species: Synthetic
Genetic Insert: C-Terminal Sensor 1 V1
Vector Backbone Description: Backbone Marker:invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-NEO-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26389572
Proper citation: RRID:Addgene_68795 Copy
Species: Homo sapiens
Genetic Insert: optineurin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24683533
Comments: Wild type construct is pOPTN-EGFP (Plasmid #27052)
Proper citation: RRID:Addgene_68841 Copy
Species: Synthetic
Genetic Insert: ZF9-VP64
Vector Backbone Description: Backbone Marker:invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-NEO-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26389572
Proper citation: RRID:Addgene_68737 Copy
Species: Synthetic
Genetic Insert: ZF8-VP64
Vector Backbone Description: Backbone Marker:invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-NEO-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26389572
Proper citation: RRID:Addgene_68736 Copy
Species: Synthetic
Genetic Insert: ZF6-VP64
Vector Backbone Description: Backbone Marker:invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-NEO-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26389572
Proper citation: RRID:Addgene_68734 Copy
Species: Synthetic
Genetic Insert: ZF5-VP64
Vector Backbone Description: Backbone Marker:invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-NEO-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26389572
Proper citation: RRID:Addgene_68733 Copy
Species: Synthetic
Genetic Insert: ZF4-VP64
Vector Backbone Description: Backbone Marker:invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-NEO-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26389572
Proper citation: RRID:Addgene_68732 Copy
Species: Synthetic
Genetic Insert: ZF6/ZF10-VP64
Vector Backbone Description: Backbone Marker:invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-NEO-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26389572
Proper citation: RRID:Addgene_68750 Copy
Species: Rattus norvegicus
Genetic Insert: Gja1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26265468
Comments: Addition of a fluorescent protein tag to the carboxyl-terminus of Cx43 has been shown to eliminate or attenuate interactions with ZO-1 and Occludin in cell culture. Other binding interactions and channel gating properties may be affected.
Proper citation: RRID:Addgene_69007 Copy
Species: Homo sapiens
Genetic Insert: optineurin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24683533
Comments: Wild type construct is pOPTN-EGFP (Plasmid #27052)
Proper citation: RRID:Addgene_68847 Copy
Species: Synthetic
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Life Technologies/Invitrogen; Backbone Size:2655; Vector Backbone:pDONRP4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23957834
Proper citation: RRID:Addgene_48350 Copy
Species: Homo sapiens
Genetic Insert: dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
Vector Backbone Description: Vector Backbone:pmax-DEST (Addgene: 48222); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48226 Copy
Species: Homo sapiens
Genetic Insert: dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
Vector Backbone Description: Vector Backbone:pmax-DEST (Addgene: 48222); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48227 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Life Technologies/Invitrogen; Backbone Size:2655; Vector Backbone:pDONRP4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23957834
Proper citation: RRID:Addgene_48348 Copy
Species: Homo sapiens
Genetic Insert: dCas9(D10A;H840A) fusion with VP96 activation domain
Vector Backbone Description: Vector Backbone:pmax-DEST (Addgene: 48222); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48224 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Backbone Marker:Life Technologies/Invitrogen; Backbone Size:2639; Vector Backbone:pDONRP2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23957834
Proper citation: RRID:Addgene_48345 Copy
Species: Homo sapiens
Genetic Insert: dCas9(D10A;H840A) fusion with VP160 activation domain
Vector Backbone Description: Vector Backbone:pmax-DEST (Addgene: 48222); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48225 Copy
Species: Synthetic
Genetic Insert: V5 and 6xHis epitope tags cassette
Vector Backbone Description: Backbone Marker:Life Technologies/Invitrogen; Backbone Size:2645; Vector Backbone:pDONRP4-P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23957834
Proper citation: RRID:Addgene_48346 Copy
Species: Homo sapiens
Genetic Insert: dCas9(D10A;H840A) fusion with VP64 activation domain
Vector Backbone Description: Vector Backbone:pmax-DEST (Addgene: 48222); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48223 Copy
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