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Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141017 Copy
Species: Synthetic
Genetic Insert: mVenus
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a K206A mutation in Venus. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_141019 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141024 Copy
Species: Synthetic
Genetic Insert: mCerulean
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141020 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141022 Copy
Species: Synthetic
Genetic Insert: mVenus
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a K206A mutation in Venus and a P349R mutation in HygTK. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_141028 Copy
Species: Synthetic
Genetic Insert: mCerulean
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31454364
Comments: Please note: Plasmid contains a single G nucleotide deletion in attB1. This deletion is not known to affect plasmid function.
Proper citation: RRID:Addgene_141029 Copy
Species: Human cytomegalovirus
Genetic Insert: CMV promoter
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141035 Copy
Species: Synthetic
Genetic Insert: CAG promoter
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141036 Copy
Species: Homo sapiens
Genetic Insert: EF1_ promoter
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141037 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141031 Copy
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141034 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pPB-MCSfw-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141030 Copy
Species: Synthetic
Genetic Insert: Csy4-2A-FokI-dCas9-2A-EGFP
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141047 Copy
Species: Synthetic
Genetic Insert: Csy4-2A-FokI-dCas9
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141042 Copy
Species: Synthetic
Genetic Insert: Csy4-2A-Cas9-2A-EGFP
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141045 Copy
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141054 Copy
Species: Synthetic
Genetic Insert: SpCas9-HF2-2A-EGFP
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141050 Copy
Species: Synthetic
Genetic Insert: eSpCas9(1.1)-2A-EGFP
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141051 Copy
Species: Synthetic
Genetic Insert: eSpCas9(1.1)-2A-EGFP
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pDONR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31454364
Proper citation: RRID:Addgene_141052 Copy
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