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Genetic Insert: Control sgRNA
Vector Backbone Description: Backbone Size:8888; Vector Backbone:pSICO derivative; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29887379
Proper citation: RRID:Addgene_128749 Copy
Species: Homo sapiens
Genetic Insert: Spastin
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX; Vector Types:Bacterial Expression, `; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31767681
Proper citation: RRID:Addgene_128794 Copy
Vector Backbone Description: Backbone Size:3733; Vector Backbone:pLZ12-Spec; Vector Types:Bacterial mutagenesis; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32430813
Proper citation: RRID:Addgene_128799 Copy
Species: Synthetic
Genetic Insert: ssGNb-mCherry
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31122229
Proper citation: RRID:Addgene_128788 Copy
Species: Mus musculus
Genetic Insert: Cep83
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24469809
Proper citation: RRID:Addgene_128874 Copy
Vector Backbone Description: Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31461461
Comments: Please visit https://www.biorxiv.org/content/10.1101/630277v1 for BioRxiv preprint.
Proper citation: RRID:Addgene_128859 Copy
Species: Mus musculus
Genetic Insert: Nuclear EYFP into Elf5 locus
Vector Backbone Description: Vector Backbone:pNTKV ; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31031139
Proper citation: RRID:Addgene_128833 Copy
Species: E. coli
Genetic Insert: rpoZ
Vector Backbone Description: Backbone Size:3939; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23389035
Proper citation: RRID:Addgene_128837 Copy
Species: Mus musculus
Genetic Insert: mElf5 gRNA
Vector Backbone Description: Vector Backbone:pX330 ; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31031139
Proper citation: RRID:Addgene_128836 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31269210
Proper citation: RRID:Addgene_129020 Copy
Species: E. coli
Genetic Insert: RpoA
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21416542
Proper citation: RRID:Addgene_128940 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: To design forward PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) ATGGTGAGTAAAGGCGAGGA-3'. To design reverse PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) GAATTCGAGCTCGTTTAAAC-3'.
Proper citation: RRID:Addgene_129100 Copy
Species: Synthetic
Genetic Insert: CherryOFF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7116; Vector Backbone:pQC-XIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29909992
Comments: Addgene plasmid # 26286 is the empty vector negative control.
Proper citation: RRID:Addgene_129101 Copy
Species: Synthetic
Genetic Insert: dCas9-hudTET1CD, SgRNA6 (huTSDR)
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Proper citation: RRID:Addgene_129046 Copy
Species: Synthetic
Genetic Insert: dCas9-huTET1CD, SgRNA cloning site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI).
This plasmid was generated by Christopher Kressler.
Proper citation: RRID:Addgene_129027 Copy
Species: Synthetic
Genetic Insert: dCas9-huTET1CD, SgRNA cloning site
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33488615
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI).
This Plasmid was generated by Christopher Kressler.
Proper citation: RRID:Addgene_129025 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: To design forward PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) ATGGTGAGTAAAGGCGAGGA-3'. To design reverse PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) GAATTCGAGCTCGTTTAAAC-3'.
Proper citation: RRID:Addgene_129099 Copy
Species: Homo sapiens
Genetic Insert: cJun
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5370; Vector Backbone:pET 29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31857650
Comments: Depositor uses BL21 for protein production.
Proper citation: RRID:Addgene_129227 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:2793; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31315929
Proper citation: RRID:Addgene_129291 Copy
Species: Synthetic
Genetic Insert: QUEEN-37C
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Depositor Adm #1042 . Please visit https://www.biorxiv.org/content/10.1101/2021.10.08.463131v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129318 Copy
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