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Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab (Addgene #37084); Backbone Size:6342; Vector Backbone:pAAV-Ef1a-DIO-EGFP-WPRE-pA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28888955
Proper citation: RRID:Addgene_91946 Copy
Species: Synthetic
Genetic Insert: MTS-mCherry-GFP1-10
Vector Backbone Description: Backbone Marker:PMID: 7737504; Vector Backbone:p404TDH3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28241148
Comments: The MTS sequence is based on subunit 9 of F0-ATP synthase (pOLI1-HcRed) from Curr Biol. 2004 Nov 23;14(22):1996-2004
Proper citation: RRID:Addgene_91958 Copy
Species: Synthetic
Genetic Insert: MTS-mCherry-GFP1-10
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pDsRed-Monomer-Hyg-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28241148
Comments: The MTS sequence is based on subunit 9 of F0-ATP synthase (pOLI1-HcRed) from Curr Biol. 2004 Nov 23;14(22):1996-2004
Proper citation: RRID:Addgene_91957 Copy
Species: Synthetic
Genetic Insert: FlucWT-HA-GFP11
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28241148
Proper citation: RRID:Addgene_91954 Copy
Species: Synthetic
Genetic Insert: GST-HA-GFP11
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28241148
Proper citation: RRID:Addgene_91953 Copy
Species: Synthetic
Genetic Insert: DsRed-IRES-EGFP
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLenti-GPS-GAW-IRES; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27779468
Proper citation: RRID:Addgene_92194 Copy
Species: Synthetic
Genetic Insert: C-BLa 2.0 fusion protein
Vector Backbone Description: Backbone Marker:John E. Cronan; Backbone Size:4284; Vector Backbone:pBAD322K (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28432015
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_92060 Copy
Species: Synthetic
Genetic Insert: ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
Vector Backbone Description: Backbone Marker:Voth et al. (2001) Nucleic Acids Res (PMID: 11410682); Backbone Size:6062; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression, Integration and expression into yeast.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989441
Comments: The original version of the ATeam1.03 FRET sensor (tested in mammalian cells but not codon optimized for yeast) was published in 2009 by Imamura et al (PMID: 19720993)
Proper citation: RRID:Addgene_92179 Copy
Species: Synthetic
Genetic Insert: i53
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4614; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29176614
Comments: bioRxiv (10.1101/060954)
Proper citation: RRID:Addgene_92170 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2530; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology, Cell Free Protein Synthesis (CFPS); Bacterial Resistance:Ampicillin
Defining Citation: PMID:28230975
Comments: Additional reference:
Shin, J., and Noireaux, V. (2010) Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. J. Biol. Eng. 4, 8.
Proper citation: RRID:Addgene_92224 Copy
Species: Synthetic
Genetic Insert: Sp-dCas9-2xAM tag
Vector Backbone Description: Backbone Marker:Yoichi Sekita; Backbone Size:9921; Vector Backbone:CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29482606
Proper citation: RRID:Addgene_92221 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2530; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology, Cell Free Protein Synthesis (CFPS); Bacterial Resistance:Ampicillin
Defining Citation: PMID:28230975
Comments: Additional reference:
Shin, J., and Noireaux, V. (2010) Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. J. Biol. Eng. 4, 8.
Proper citation: RRID:Addgene_92222 Copy
Species: Synthetic
Genetic Insert: TALEN
Vector Backbone Description: Backbone Size:5819; Vector Backbone:pCAG-ZEO-IRES; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811996
Proper citation: RRID:Addgene_92245 Copy
Species: Synthetic
Genetic Insert: TALEN
Vector Backbone Description: Backbone Size:5843; Vector Backbone:pCAG-BSD-IRES; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811996
Proper citation: RRID:Addgene_92246 Copy
Species: Synthetic
Genetic Insert: TALEN
Vector Backbone Description: Backbone Size:5055; Vector Backbone:pcDNA4/myc-hisA; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811996
Proper citation: RRID:Addgene_92243 Copy
Species: Synthetic
Genetic Insert: Neuron-astrocyte proximity assay - Neuronal
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621490
Proper citation: RRID:Addgene_92278 Copy
Species: Synthetic
Genetic Insert: Neuron-astrocyte proximity assay - Astrocyte
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29621490
Proper citation: RRID:Addgene_92277 Copy
Species: Synthetic
Genetic Insert: Ancestral AAV Capsid Anc80L65AAP
Vector Backbone Description: Backbone Size:5238; Vector Backbone:synthetic; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28165475
Proper citation: RRID:Addgene_92307 Copy
Species: Synthetic
Genetic Insert: CaM-V5-TEVp(Δ220-242)
Vector Backbone Description: Backbone Size:3659; Vector Backbone:AAV vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650461
Proper citation: RRID:Addgene_92214 Copy
Species: Synthetic
Genetic Insert: NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
Vector Backbone Description: Backbone Size:3659; Vector Backbone:AAV vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28650461
Proper citation: RRID:Addgene_92213 Copy
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