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Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9660945
Comments: Two copies of the Smad binding site (taaGTCTAGACggcaGTCTAGACgtac) are cloned into pGL3.
Proper citation: RRID:Addgene_16500 Copy
Species: Synthetic
Genetic Insert: POLArISact
Vector Backbone Description: Backbone Marker:Zhao et. al. DOI: 10.1126/science.1208592; Vector Backbone:modified pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33674463
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.20.116178v2 full for bioRxiv preprint.
Proper citation: RRID:Addgene_164971 Copy
Species: Homo sapiens
Genetic Insert: APC
Vector Backbone Description: Backbone Marker:Vogelstein lab (Addgene plasmid# 16440); Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Proper citation: RRID:Addgene_16507 Copy
Species: SARS-COV-2
Genetic Insert: Nsp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165108 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pcDNA?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
Proper citation: RRID:Addgene_16512 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: COV E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165123 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF3a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165121 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: ORF9b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165122 Copy
Species: Homo sapiens
Genetic Insert: OCT4
Vector Backbone Description: Vector Backbone:pMaster; Vector Types:Mammalian Expression, Episomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088009
Comments: Please note that Addgene's NGS found that the bGH poly(A) signal between one of the oris and Lin28 is absent as compared to the annotated sequence provided by the depositor.
Please note that this plasmid contains repetitive elements which are prone to recombination. Screen 3-5 small colonies to ensure the full plasmid is selected. For example, the restriction enzyme NheI can be used to check the plasmid size as shown in Addgene's Diagnostic Digest.
Proper citation: RRID:Addgene_165081 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp15
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165120 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165112 Copy
Species: Ruminoccocus flavefaciens XPD3002
Genetic Insert: RfxCas13d
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Proper citation: RRID:Addgene_165077 Copy
Species: Other
Genetic Insert: Scaffold gRNA
Vector Backbone Description: Vector Backbone:lentiGuide-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Comments: Please note that viral vectors are prone to recombination. Addgene recommends testing 2-4 individual colonies to ensure the full plasmid is intact.
Proper citation: RRID:Addgene_165078 Copy
Species: Ruminoccocus flavefaciens XPD3002
Genetic Insert: RfxCas13d
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33288960
Comments: Please note that the fluorophore is sfGFP. Previous versions of SnapGene may label it as mCFP.
Proper citation: RRID:Addgene_165076 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp7
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165134 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 spike protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165096 Copy
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Comments: SBE-luc was made by annealing the oligonucleotides 5′-CGGAGTATGTCTAGACTGACAATG-3′ and 5′-CTAGCATTGTCAGTCTAGACATACTCCGAGCT-3′ and cloning them into the Sac1 and Nhe1 sites.
Proper citation: RRID:Addgene_16527 Copy
Species: Other
Genetic Insert: EGFP-NLS
Vector Backbone Description: Backbone Marker:Roger Tsien; Vector Backbone:AAV-flex-ReaChR-citrine (Addgene 50955); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34176926
Proper citation: RRID:Addgene_165441 Copy
Species: Homo sapiens
Genetic Insert: FAST-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702198
Comments: To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.
Proper citation: RRID:Addgene_16521 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Comments: HCT116 has a 3-bp deletion that removes one amino acid (Ser45).
Proper citation: RRID:Addgene_16520 Copy
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