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Species: Caenorhabditis elegans
Genetic Insert: EGFP^SEC^3xFlag
Vector Backbone Description: Backbone Size:3854; Vector Backbone:pDD282; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32586975
Proper citation: RRID:Addgene_75027 Copy
Species: Danio rerio
Genetic Insert: ELAV like neuron-specific RNA binding protein 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2763; Vector Backbone:pDONRP2R-P3; Vector Types:5' entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75025 Copy
Species: Staphylococcus aureus
Genetic Insert: reprogrammed sortase A variant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25187567
Comments: eSrtA(4S-9) has 120 fold change in substrate specificity with activity comparable to eSrtA and it prefers LPXSG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).
Proper citation: RRID:Addgene_75146 Copy
Species: Staphylococcus aureus
Genetic Insert: reprogrammed sortase A variant
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25187567
Comments: eSrtA(2A-9) has 51,000 fold change in substrate specificity with activity comparable to eSrtA and it prefers LAXTG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).
Proper citation: RRID:Addgene_75145 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP2^SEC^3xFlag
Vector Backbone Description: Backbone Size:3854; Vector Backbone:pDD282; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_75029 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry^SEC^3xFlag
Vector Backbone Description: Backbone Size:3854; Vector Backbone:pDD282; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32586975
Proper citation: RRID:Addgene_75028 Copy
Species: Synthetic
Genetic Insert: mTagBFP-CAAX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2550; Vector Backbone:pDONR221; Vector Types:Middle entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75149 Copy
Species: Homo sapiens
Genetic Insert: ETV5
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Backbone Size:9335; Vector Backbone:pLKO.5; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28178529
Proper citation: RRID:Addgene_74977 Copy
Species: Mus musculus
Genetic Insert: interleukin 1 beta
Vector Backbone Description: Backbone Size:6300; Vector Backbone:p3XFLAG-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26642356
Comments: Please note that mutation E130K in Il1b was found during Addgene's quality control. The depositor noted that this mutation is expected and does NOT affect its cleavage by caspase 1.
Proper citation: RRID:Addgene_75131 Copy
Species: Mus musculus
Genetic Insert: shBrd9_1116
Vector Backbone Description: Vector Backbone:MSCV-miR30-shRNA-PGK-NeoR-IRES-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75130 Copy
Species: Mus musculus
Genetic Insert: NLR family, pyrin domain containing 3
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26642356
Proper citation: RRID:Addgene_75137 Copy
Species: Homo sapiens
Genetic Insert: shBRD9_508
Vector Backbone Description: Vector Backbone:MSCV-miRE- shRNA-SV40-GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27376689
Proper citation: RRID:Addgene_75138 Copy
Species: Synthetic
Genetic Insert: hM4D(Gi)-mCherry
Vector Backbone Description: Backbone Size:4023; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247388
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_75033 Copy
Genetic Insert: ETV4
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Backbone Size:10065; Vector Backbone:pLX_TRC311; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28178529
Proper citation: RRID:Addgene_74983 Copy
Species: Homo sapiens
Genetic Insert: ETV4
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Backbone Size:10065; Vector Backbone:pLX_TRC311; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28178529
Proper citation: RRID:Addgene_74982 Copy
Species: Homo sapiens
Genetic Insert: ETV1
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Backbone Size:10065; Vector Backbone:pLX_TRC311; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28178529
Proper citation: RRID:Addgene_74981 Copy
Species: Homo sapiens
Genetic Insert: ETV5
Vector Backbone Description: Backbone Marker:Broad Institute - Genetic Perturbation Platform; Backbone Size:10065; Vector Backbone:pLX_TRC311; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28178529
Proper citation: RRID:Addgene_74984 Copy
Species: Rattus norvegicus
Genetic Insert: ATP2A1
Vector Backbone Description: Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8447366
Proper citation: RRID:Addgene_75182 Copy
Species: Homo sapiens
Genetic Insert: ATP2A2
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2844796
Proper citation: RRID:Addgene_75187 Copy
Species: Synthetic
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2666; Vector Backbone:pDONRP4P1R; Vector Types:Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen); Bacterial Resistance:Kanamycin
Defining Citation: PMID:27631880
Proper citation: RRID:Addgene_75175 Copy
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