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Species: Mus musculus
Genetic Insert: Pyruvate Kinase M1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337823
Proper citation: RRID:Addgene_44240 Copy
Species: Mus musculus
Genetic Insert: Pyruvate Kinase M2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6800; Vector Backbone:pLHCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18337823
Proper citation: RRID:Addgene_44239 Copy
Species: Homo sapiens
Genetic Insert: EF1a-GFP
Vector Backbone Description: Backbone Marker:LJ Chang; Backbone Size:8738; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20517486
Proper citation: RRID:Addgene_44352 Copy
Species: Synthetic
Genetic Insert: Laconic (LACtate Optical Nano Indicator from CECs)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5418; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression, lactate biosensor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23469056
Comments: Laconic is a genetically-encoded biosensor that detects lactate in the physiological range. The sensor was based on LldR, a bacterial transcription regulator that consists of a lactate-binding/regulatory domain and a DNA-binding domain. Laconic consists of a N-terminal mTFP, followed by the LldR flanked by linkers, and finally a C-terminal Venus.
See Addgene plasmid# 46307: NLS-Laconic/pCMV-myc-nuc for a nuclear-targeted version of Laconic: http://www.addgene.org/46307/ .
Proper citation: RRID:Addgene_44238 Copy
Species: Homo sapiens
Genetic Insert: PP1B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11739654
Comments: Please note that there is a silent mutation at bp#842 when compared to GenBank reference sequence NM_002709.2.
Proper citation: RRID:Addgene_44223 Copy
Species: Homo sapiens
Genetic Insert: PP-1G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11739654
Comments: Please note that there is a silent mutation at bp#784 when compared to GenBank reference sequence NM_002710.3.
Proper citation: RRID:Addgene_44225 Copy
Species: Synthetic
Genetic Insert: CBF1-Responsive-Element
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4540; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23617465
Proper citation: RRID:Addgene_44211 Copy
Species: Homo sapiens
Genetic Insert: RepoMan
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16492807
Proper citation: RRID:Addgene_44213 Copy
Species: Pea
Genetic Insert: Connexin43 fused to pea APEX
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23086203
Proper citation: RRID:Addgene_44439 Copy
Vector Backbone Description: Backbone Size:4850; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15644491
Proper citation: RRID:Addgene_44433 Copy
Species: Vibrio cholerae
Genetic Insert: AphA
Vector Backbone Description: Backbone Marker:AATC; Backbone Size:8807; Vector Backbone:pMMB66EH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23919997
Proper citation: RRID:Addgene_44392 Copy
Species: Homo sapiens
Genetic Insert: mitoDsRed
Vector Backbone Description: Backbone Size:6727; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314018
Comments: Limitations of allotopic expression of mitochondrial genes in mammalian cells. Oca-Cossio J, Kenyon L, Hao H, Moraes CT.
Genetics. 2003 Oct;165(2):707-20. PMID: 14573482
Proper citation: RRID:Addgene_44386 Copy
Species: Homo sapiens
Genetic Insert: mitoGFP
Vector Backbone Description: Backbone Size:6727; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314018
Proper citation: RRID:Addgene_44385 Copy
Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1, NeoR, peel-1, MCS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2305; Vector Backbone:pDESTR4-R3; Vector Types:Multiple cloning site vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44484 Copy
Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2445; Vector Backbone:pDESTR4-R3; Vector Types:Gateway expression clone; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44489 Copy
Species: Drosophila melanogaster
Genetic Insert: 500bp Mos1, unc-119, fosmid cassette
Vector Backbone Description: Backbone Size:1682; Vector Backbone:Unknown; Vector Types:Fosmid recombineering cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44488 Copy
Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1, NeoR, MCS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2305; Vector Backbone:pDESTR4-R3; Vector Types:Multiple cloning site vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44481 Copy
Species: Homo sapiens
Genetic Insert: hM4D(Gi)-mCherry
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pAAV; Vector Types:AAV, Adeno Associated Viral Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21364278
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_44362 Copy
Species: Mus musculus
Genetic Insert: Notch intracellular domain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21131365
Proper citation: RRID:Addgene_44471 Copy
Species: Synthetic
Genetic Insert: QQR ZFN
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: QQR ZFN reference:
Even-Faitelson L, Samach A, Melamed-Bessudo C, Avivi-Ragolsky N, Levy AA (2011) Localized egg-cell expression of effector proteins for targeted modification of the Arabidopsis genome. The Plant Journal 68 (5):929-937. doi:10.1111/j.1365-313X.2011.04741.x
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44463 Copy
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