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Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31527135
Comments: Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_133759 Copy
Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:plvx-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31527135
Comments: Please visit https://www.biorxiv.org/content/10.1101/560144v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_133758 Copy
Species: Homo sapiens
Genetic Insert: Wild type EGFR-HA
Vector Backbone Description: Backbone Size:10455; Vector Backbone:PWPXLD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31433979
Proper citation: RRID:Addgene_133749 Copy
Species: Homo sapiens
Genetic Insert: CSF1-R
Vector Backbone Description: Backbone Size:6415; Vector Backbone:MSCV LTR ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3027579
Proper citation: RRID:Addgene_133744 Copy
Species: Homo sapiens
Genetic Insert: SON Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human SON in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_133964 Copy
Species: Homo sapiens
Genetic Insert: UBTF Homology Arms with HaloTag-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a HaloTag to the N-terminus of human UBTF in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SG. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_133963 Copy
Species: Homo sapiens
Genetic Insert: RanGAP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pDsRed1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11854305
Proper citation: RRID:Addgene_13386 Copy
Species: Homo sapiens
Genetic Insert: LMNA (Lam)
Vector Backbone Description: Backbone Size:5846; Vector Backbone:pBKWH; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_133899 Copy
Species: Homo sapiens
Genetic Insert: HPS4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCINeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12756248
Proper citation: RRID:Addgene_133931 Copy
Species: Homo sapiens
Genetic Insert: HPS1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCINeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12756248
Proper citation: RRID:Addgene_133930 Copy
Species: Homo sapiens
Genetic Insert: EGFP and MLC2(CA)
Vector Backbone Description: Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31679934
Proper citation: RRID:Addgene_133928 Copy
Species: Homo sapiens
Genetic Insert: EB3-mCherry and Lifeact-GFP
Vector Backbone Description: Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31679934
Proper citation: RRID:Addgene_133927 Copy
Species: Homo sapiens
Genetic Insert: 6 copies of the AP-1 binding motif
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:3057; Vector Backbone:pUPD; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32539183
Comments: Insert can be released with BsaI
Proper citation: RRID:Addgene_133884 Copy
Species: Homo sapiens
Genetic Insert: SUMO-conjugating enzyme UBC9
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929246
Proper citation: RRID:Addgene_133911 Copy
Species: Homo sapiens
Genetic Insert: SUMO-conjugating enzyme UBC9
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32929246
Proper citation: RRID:Addgene_133910 Copy
Species: Homo sapiens
Genetic Insert: UtrCH
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5373; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31264686
Proper citation: RRID:Addgene_133875 Copy
Species: Homo sapiens
Genetic Insert: UtrCH
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5373; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31264686
Proper citation: RRID:Addgene_133874 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin-fold modifier 1 lacking last three C-terminal residues
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31595041
Proper citation: RRID:Addgene_133866 Copy
Species: Homo sapiens
Genetic Insert: CD86
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31606123
Proper citation: RRID:Addgene_133860 Copy
Species: Homo sapiens
Genetic Insert: GNG2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31606123
Proper citation: RRID:Addgene_133857 Copy
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