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Species: Homo sapiens
Genetic Insert: single chain-Fv, scFv
Vector Backbone Description: Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin
Comments: Target NCBI gene ID: 2521
Proper citation: RRID:Addgene_166561 Copy
Species: Homo sapiens
Genetic Insert: single chain-Fv, scFv
Vector Backbone Description: Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin
Comments: Target NCBI gene ID: 9255
Proper citation: RRID:Addgene_166560 Copy
Species: Synthetic
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166606 Copy
Species: Synthetic
Genetic Insert: EGFP-2A-Tetanus Toxin
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166603 Copy
Species: Synthetic
Genetic Insert: rtTA
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166598 Copy
Species: Synthetic
Genetic Insert: tTA
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166597 Copy
Genetic Insert: mTn7::Gateway cassette
Vector Backbone Description: Backbone Size:14263; Vector Backbone:pGRG37; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16646962
Comments: Grow at 32'C. Contains Gateway cassette with ccdB gene. See author's protocol for more information.
This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.
Proper citation: RRID:Addgene_16667 Copy
Genetic Insert: mTn7::MCS
Vector Backbone Description: Backbone Size:12549; Vector Backbone:pGRG36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16646962
Comments: Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information.
This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.
Proper citation: RRID:Addgene_16666 Copy
Species: Synthetic
Genetic Insert: hM3Dq-mCherry
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166599 Copy
Genetic Insert: mTn7::MCS
Vector Backbone Description: Backbone Size:12546; Vector Backbone:pGRG25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16646962
Comments: Grow at 32'C. Same as pGRG36 except for the MCS. See author's protocol for more information.
This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.
Proper citation: RRID:Addgene_16665 Copy
Species: Homo sapiens
Genetic Insert: single chain-Fv, scFv
Vector Backbone Description: Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin
Comments: Target NCBI gene ID: 23476
Proper citation: RRID:Addgene_166518 Copy
Species: Homo sapiens
Genetic Insert: single chain-Fv, scFv
Vector Backbone Description: Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin
Comments: Target NCBI gene ID: 6597
Proper citation: RRID:Addgene_166519 Copy
Vector Backbone Description: Backbone Size:7179; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD133.51, Ligation number L4664.
Proper citation: RRID:Addgene_1666 Copy
Species: Synthetic
Genetic Insert: ActA tail anchor (Listeria monocytogenes)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: "SGLRSRGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDARGSGLRSRAQASNSAVDAR
GSGLRSRAQASNSAVDARV" amino acid targeting sequence, which we refer to as "ActA", for the mitochondrial outer membrane. Pearson's correlation of 0.8-0.9 with mitotracker red signal in cells. "79aa" indicates the 79 amino acid flexible linker region between Venus and the ActA targeting signal.
Proper citation: RRID:Addgene_166763 Copy
Species: Homo sapiens
Genetic Insert: BimEL(Mus musculus)-(Noxa BH3)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the β-barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore.
Proper citation: RRID:Addgene_166761 Copy
Species: Homo sapiens
Genetic Insert: NEDD4L W576A
Vector Backbone Description: Backbone Size:5842; Vector Backbone:pCI-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166710 Copy
Species: Homo sapiens
Genetic Insert: AAVR-FLAG
Vector Backbone Description: Backbone Marker:addgene plasmid #17452; Backbone Size:9628; Vector Backbone:pLenti-CMV-Puro-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26814968
Proper citation: RRID:Addgene_166716 Copy
Species: Homo sapiens
Genetic Insert: Angiomotin p130 L106P
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34284061
Proper citation: RRID:Addgene_166714 Copy
Species: Lachnospiraceae bacterium C6A11
Genetic Insert: LahT150
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30638446
Proper citation: RRID:Addgene_166785 Copy
Genetic Insert: Spike receptor binding domain (RBD) from SARS-CoV-2
Vector Backbone Description: Backbone Marker:Andrew Scharenberg; Vector Backbone:pETcon(-) (Plasmid #41522); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32841599
Proper citation: RRID:Addgene_166782 Copy
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