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Species: Synthetic
Genetic Insert: MSP1D1Q1
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pET 28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_97326 Copy
Species: Synthetic
Genetic Insert: sfGFP1-10OPT
Vector Backbone Description: Vector Backbone:pCAMBIA1380; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28619883
Comments: Addgene’s sequencing results found an insertion of IS4 transposase in this vector not annotated in the original sequence. The depositor has verified that this vector is functional for target gene expression.
Proper citation: RRID:Addgene_97387 Copy
Species: Synthetic
Genetic Insert: nucHCB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:peYFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28017939
Proper citation: RRID:Addgene_97424 Copy
Species: Synthetic
Genetic Insert: ER-sfCherry1-10
Vector Backbone Description: Vector Backbone:pCAMBIA1380; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28619883
Proper citation: RRID:Addgene_97403 Copy
Species: Synthetic
Genetic Insert: mCherry-sfGFP11
Vector Backbone Description: Vector Backbone:pCAMBIA1380; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28619883
Proper citation: RRID:Addgene_97402 Copy
Species: Synthetic
Genetic Insert: ER-sfYFP1-10
Vector Backbone Description: Vector Backbone:pCAMBIA1380; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28619883
Proper citation: RRID:Addgene_97404 Copy
Species: Synthetic
Genetic Insert: ER-GUS-sfCherry11
Vector Backbone Description: Vector Backbone:pCAMBIA1380; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28619883
Proper citation: RRID:Addgene_97406 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29507411
Proper citation: RRID:Addgene_98035 Copy
Species: Synthetic
Genetic Insert: mCre
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29507411
Proper citation: RRID:Addgene_98034 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29507411
Proper citation: RRID:Addgene_98036 Copy
Species: Synthetic
Genetic Insert: sfGFP1-10OPT
Vector Backbone Description: Vector Backbone:pCAMBIA1380; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28619883
Proper citation: RRID:Addgene_97392 Copy
Species: Synthetic
Genetic Insert: PRPBSA-driven PuroR expression
Vector Backbone Description: Backbone Size:3105; Vector Backbone:pTS1107 (pUC57-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33857582
Proper citation: RRID:Addgene_169639 Copy
Species: Synthetic
Genetic Insert: sYFP2
Vector Backbone Description: Vector Backbone:pGRG36; Vector Types:Bacterial Expression, miniTn7 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118559 Copy
Species: Synthetic
Genetic Insert: CF-mCherry
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35179286
Proper citation: RRID:Addgene_183166 Copy
Species: Synthetic
Genetic Insert: cmv-mCherry-10xPP7
Vector Backbone Description: Backbone Size:4718; Vector Backbone:pKAN; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185796 Copy
Species: Synthetic
Genetic Insert: cmv-mCherry-8xPP7
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pKAN; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185795 Copy
Species: Synthetic
Genetic Insert: sfGFP-GB1-scAB
Vector Backbone Description: Backbone Size:5365; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185794 Copy
Species: Synthetic
Genetic Insert: humanized ChR2-H134R (partial)
Vector Backbone Description: Backbone Marker:Edward Boyden; Backbone Size:6150; Vector Backbone:AAVrg-CAG-tdTomato-WPRE-SV40 (Addgene, Plasmid #59462); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35296859
Proper citation: RRID:Addgene_180784 Copy
Species: Synthetic
Genetic Insert: pcp-12xSunTag
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185799 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
Vector Backbone Description: Backbone Size:6311; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35990749
Comments: This plasmid is based on the pUCmini-iCAP-PHP.eB plasmid. Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_184592 Copy
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