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Species: Mus musculus
Genetic Insert: Folliculin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5400; Vector Backbone:ptdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24081491
Proper citation: RRID:Addgene_49174 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49323 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49322 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24116091
Proper citation: RRID:Addgene_49321 Copy
Species: Other
Genetic Insert: AND gate
Vector Backbone Description: Vector Backbone:pEXT20; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24316737
Comments: AND gate controlled by Ptac and Ptet input promoters.
Proper citation: RRID:Addgene_49377 Copy
Species: Bacillus subtilis
Genetic Insert: LmrA repressor
Vector Backbone Description: Vector Backbone:pCI-YFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24316737
Comments: LmrA controlled by Plux promoter
Proper citation: RRID:Addgene_49375 Copy
Species: Synthetic
Genetic Insert: ntdTomato
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25643149
Comments: Please note that Addgene NGS results identified as stop codon after the second EGFP ORF. This stop codon was present in the original, published version of the plasmid, such that the insert is n2GFP rather than n3GFP. The plasmid functions as described in the associated publication. Co-expression with the helper plasmid pSOUP is suggested for expression in Agrobacterium.
The diagnostic digest indicates this plasmid may be bigger than the assembled sequence suggests.
Proper citation: RRID:Addgene_61628 Copy
Species: Homo sapiens
Genetic Insert: Cezanne2
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: This construct is the Isoform 2 of Cezanne2.
Proper citation: RRID:Addgene_61582 Copy
Genetic Insert: SNAP-EF(N)-5nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61659 Copy
Genetic Insert: SNAP-EF(N)-60nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61663 Copy
Genetic Insert: SNAP-EF(N)-10nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61660 Copy
Genetic Insert: SNAP-EF(N)-30nmSAH-GFP(1-10)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Comments: GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
Proper citation: RRID:Addgene_61662 Copy
Genetic Insert: mEGFP-30nmSAH-IQ
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61678 Copy
Genetic Insert: mEGFP-EF(N)-30nmSAH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61679 Copy
Species: Rattus norvegicus
Genetic Insert: KIF5C(1-560)-EF(C)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61675 Copy
Species: Rattus norvegicus
Genetic Insert: KHC(1-560)-mCitrine
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61676 Copy
Vector Backbone Description: Backbone Size:4658; Vector Backbone:pBAMD1-2; Vector Types:Synthetic Biology, Mini-Tn5 vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25389526
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_61564 Copy
Genetic Insert: LSSmOrange and CNA35
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25490719
Proper citation: RRID:Addgene_61605 Copy
Species: Rattus norvegicus
Genetic Insert: NES-jRCaMP1a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27011354
Proper citation: RRID:Addgene_61562 Copy
Species: Rattus norvegicus
Genetic Insert: NES-jRGECO1a
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3955; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27011354
Proper citation: RRID:Addgene_61563 Copy
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