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Species: Homo sapiens
Genetic Insert: Human histone H2B
Vector Backbone Description: Backbone Marker:-; Backbone Size:8023; Vector Backbone:pRRL-cPPT-hPGKTMPrtTA-WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29944140
Comments: ABOUT BACKBONE:
The lentiviral backbone was originally designed and constructed by Barde I et al. (Barde I et al. Mol Ther. 2006. 13(2): 382-90. PMID: 16275162).
ABOUT TEST DIGESTION:
Since Xba I restriction site is lost during cloning, we recommend checking the plasmid using the enzymes: Xho I and Nhe I. You should get two fragments around 1600 bp (insert+vector) and 7500 bp (vector).
Proper citation: RRID:Addgene_165494 Copy
Genetic Insert: ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab; Vector Backbone:pB1H2_UV2-ω-dCas9::K2_UV5-sgRNA; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33441553
Proper citation: RRID:Addgene_165606 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACCT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CCTGTGATAG CAACAGATCA CCTGGCTGCC ATCGCCCCTC-3' [Oligo C , for mutant MBS1-3];
5'-ATGAATTCCG GACGTTCTGG GCAGGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCCAGG-3' [Oligo D, for mutant MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16565 Copy
Vector Backbone Description: Backbone Size:4699; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD130.04, Ligation number L4506.
Proper citation: RRID:Addgene_1658 Copy
Species: Homo sapiens
Genetic Insert: c-MYC binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: To generate reporter constructs, the following oligonucleotides were used:
5'-CCGGTACCGG GTTGTGGCAG CCAGTCACGT GCCCGCCGCG TAGCCACACC TCTGCTCCTC AGAGCAATGT CAAGCGGTCA CGTGTGATAG CAACAGATCA CGTGGCTGCC ATCGCCCCTC-3' [Oligo A, for wild-type c-MYC binding sites (MBS)1-3]; 5'-ATGAATTCCG GACGTTCTGG GCACGTGACC GCCACCCATG CGCTGAGGGG CGGACAGGAG GTGCTTCGAC TGGGAGGAGG GCGAAGAGTG TAAGGGGGCG GAGGGGCGAT GGCAGCC-3' [Oligo B, for wild-type MBS4].
Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
Proper citation: RRID:Addgene_16564 Copy
Species: Homo sapiens
Genetic Insert: BAX knockout
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11062132
Comments: This targeting construct consists of two homologous arms and a hygromycin-resistant gene.
Proper citation: RRID:Addgene_16575 Copy
Species: Homo sapiens
Genetic Insert: TCF-4 binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10749138
Comments: TCF-4 mutant binding sequence - CCTTTGGC
Proper citation: RRID:Addgene_16559 Copy
Species: Homo sapiens
Genetic Insert: MADMYC
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10688915
Comments: MADMYC: the amino
terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional
repression domain (aa 1-38, the Sin3-interaction region) of Mad.
Proper citation: RRID:Addgene_16557 Copy
Species: Viral
Genetic Insert: SR39TK
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pUPD2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34525356
Comments: CDS
Proper citation: RRID:Addgene_165841 Copy
Species: Homo sapiens
Genetic Insert: BAX alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Proper citation: RRID:Addgene_16587 Copy
Species: P1 bacteriophage
Genetic Insert: cre recombinase
Vector Backbone Description: Backbone Size:7700; Vector Backbone:na; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11371342
Comments: Cre expression vector.
Proper citation: RRID:Addgene_16583 Copy
Species: Other
Genetic Insert: HCoV-OC43 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166015 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166019 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166018 Copy
Species: Other
Genetic Insert: HCoV-229E S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166016 Copy
Species: Other
Genetic Insert: SARS-CoV-2 S (spike)
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166010 Copy
Species: Homo sapiens
Genetic Insert: PFAS-mCherry
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32439803
Proper citation: RRID:Addgene_166024 Copy
Species: Homo sapiens
Genetic Insert: p53 binding sites
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463391
Comments: The following oligo pairs containing two copies of wild-type p53 binding sites were used: 5'-CTAGGCTGCA AGTCCTGACT TGTCCACACT CTGCAAGTCC TGACTTGTCC-3' and 5'-CTAGGGACAA GTCAGGACTT GCAGAGTGTG GACAAGTCAG GACTTGCAGC-3'. The annealed oligonucleotide pairs were concatamerized and cloned into the NheI site of pBVLuc.
Proper citation: RRID:Addgene_16593 Copy
Species: Other
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_166020 Copy
Species: Xanthophyllomyces dendrorhous
Genetic Insert: Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33758180
Proper citation: RRID:Addgene_165977 Copy
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