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Species: Synthetic
Genetic Insert: U6.gRNAS1 cassette
Vector Backbone Description: Backbone Size:6930; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58252 Copy
Species: Synthetic
Genetic Insert: U6.gRNAGFP cassette
Vector Backbone Description: Backbone Size:7043; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58255 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Size:4941; Vector Backbone:HSC1 retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23752310
Comments: Please note that there is a small deletion (32bp) just after the 3’LTR inside the cloning vector that removes the M13R primer region. The depositing lab was able to produce viruses with good titer and doesn’t think the missing 32bp is a major concern.
Proper citation: RRID:Addgene_58254 Copy
Vector Backbone Description: Vector Backbone:pAPIC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30157423
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/.
Proper citation: RRID:Addgene_58375 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn2beta AS4(-)
Vector Backbone Description: Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / Bsu36I).
Insert can also be released by single digest using XhoI.
The rat neurexin 2 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58268 Copy
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847673
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_58301 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1beta AS4(-)
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / KpnI).
Insert can also be released by single digest using XhoI.
The rat neurexin 1 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the region homologous to alternatively spliced segment 5 (AS5) of mouse neurexin 1 beta (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58267 Copy
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847673
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_58300 Copy
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847673
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_58303 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58305 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58304 Copy
Species: Synthetic
Genetic Insert: Genomic cassette
Vector Backbone Description: Vector Backbone:SEVA; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24847673
Proper citation: RRID:Addgene_58307 Copy
Species: Homo sapiens
Genetic Insert: Neuroserpin
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24581498
Proper citation: RRID:Addgene_58260 Copy
Species: Homo sapiens
Genetic Insert: FADD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8519; Vector Backbone:pLVX-IRES-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24581498
Proper citation: RRID:Addgene_58263 Copy
Species: Rattus norvegicus
Genetic Insert: Nrxn1alpha
Vector Backbone Description: Backbone Size:4869; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360903
Comments: See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest).
The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]).
Note: HA-epitope tag was introduced at the mature N-terminus.
Proper citation: RRID:Addgene_58266 Copy
Species: Homo sapiens
Genetic Insert: PITP beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20332109
Comments: Addgene QC sequencing has found some mutations in the insert, which should not effect the function of the plasmid.
Proper citation: RRID:Addgene_58279 Copy
Species: Mus musculus
Genetic Insert: TNF receptor associated factor 4
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23760265
Proper citation: RRID:Addgene_58316 Copy
Species: Mus musculus
Genetic Insert: TNF receptor associated factor 5
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23760265
Proper citation: RRID:Addgene_58315 Copy
Species: Homo sapiens
Genetic Insert: PITP beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18636990
Proper citation: RRID:Addgene_58271 Copy
Species: Homo sapiens
Genetic Insert: FKBP-targeted miRNA
Vector Backbone Description: Vector Backbone:inducible knockdown/cDNA RMCE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24637323
Proper citation: RRID:Addgene_58280 Copy
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