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Species: Synthetic
Genetic Insert: mCherry-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_186347 Copy
Species: Synthetic
Genetic Insert: mCherry-3xNLS
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_186346 Copy
Species: Synthetic
Genetic Insert: EF1a
Vector Backbone Description: Backbone Marker:Addgene (Plasmid #25999); Vector Backbone:LV-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35210624
Proper citation: RRID:Addgene_186627 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-eDHFR(69K6)
Vector Backbone Description: Backbone Marker:Dr. Hiroyuki Miyoshi (RIKEN, Japan); Backbone Size:10345; Vector Backbone:pCSII-EF-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35835118
Comments: The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178853 Copy
Species: Synthetic
Genetic Insert: cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
Vector Backbone Description: Vector Backbone:pHAGE-UbiC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35784652
Proper citation: RRID:Addgene_185802 Copy
Species: Synthetic
Genetic Insert: 22aa-eDHFR(69K6)-EGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4695; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35835118
Comments: The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172101 Copy
Species: Synthetic
Genetic Insert: MTOR
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183311 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:7456; Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35294338
Proper citation: RRID:Addgene_187482 Copy
Species: Synthetic
Genetic Insert: AAV-cMG capsid
Vector Backbone Description: Vector Backbone:AAV helper vector; Vector Types:AAV, helper vector for packaging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35879607
Proper citation: RRID:Addgene_184539 Copy
Species: Synthetic
Genetic Insert: histone H2B fused with miniTurbo (BirA mutant)
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35778550
Proper citation: RRID:Addgene_187178 Copy
Species: Synthetic
Genetic Insert: miniTurbo (BirA mutant)
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35778550
Proper citation: RRID:Addgene_187176 Copy
Species: Synthetic
Genetic Insert: sfGFP, tdTomato
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Dre/Rox; Bacterial Resistance:Ampicillin
Comments: cre → sfGFP expression. dre → tdTomato expression.
Expression of sfGFP and tdTomato are independent of each other. "OR."
Actual mouse was not generated.
The EMCV IRES fragment from Dr. Peter Mombaerts is the same EMCV IRES used in the Id1-IRES-creERT2 knock-in mouse line (http://www.informatics.jax.org/allele/MGI:4366863).
Proper citation: RRID:Addgene_185966 Copy
Species: Synthetic
Genetic Insert: sfGFP, tdTomato
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Dre/Rox; Bacterial Resistance:Ampicillin
Comments: cre → sfGFP expression. cre and dre → sfGFP and tdTomato expression. dre only (no cre) → no sfGFP nor tdTomato expression.
The actual mouse did not have any abnormalities.
Proper citation: RRID:Addgene_185965 Copy
Species: Synthetic
Genetic Insert: SNAP-ecDHFR
Vector Backbone Description: Vector Backbone:pET51b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32762146
Proper citation: RRID:Addgene_187108 Copy
Species: Synthetic
Genetic Insert: EGFP, tdTomato
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Dre/Rox; Bacterial Resistance:Ampicillin
Comments: cre → EGFP expression. dre → tdTomato expression.
Expression of EGFP and tdTomato are independent of each other. "OR."
The actual mouse had a "phenotype" - the males and females were fine otherwise, but the females did not nurse the pups.
Proper citation: RRID:Addgene_185963 Copy
Species: Synthetic
Genetic Insert: rHCaMP
Vector Backbone Description: Vector Backbone:pCDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32762146
Proper citation: RRID:Addgene_187107 Copy
Species: Synthetic
Genetic Insert: AAV-cMG.WPP capsid
Vector Backbone Description: Vector Backbone:AAV helper vector; Vector Types:AAV, helper vector for packaging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35879607
Proper citation: RRID:Addgene_184542 Copy
Species: Synthetic
Genetic Insert: AAV-cMG.QRP capsid
Vector Backbone Description: Vector Backbone:AAV helper vector; Vector Types:AAV, helper vector for packaging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35879607
Proper citation: RRID:Addgene_184543 Copy
Species: Synthetic
Genetic Insert: AAV-MG1.2 capsid
Vector Backbone Description: Vector Backbone:AAV helper vector; Vector Types:AAV, helper vector for packaging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35879607
Proper citation: RRID:Addgene_184541 Copy
Species: Synthetic
Genetic Insert: FLT4
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183294 Copy
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