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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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T4 Lysozyme R52V N53A T54S N55G V57T WT* Resource Report Resource Website |
RRID:Addgene_18348 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | L32T T34K K35V S36D P37G S38N L39S WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme I58M WT* Resource Report Resource Website |
RRID:Addgene_18360 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | I58M WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme N53A N55A V57A E128A V131A N132A Resource Report Resource Website |
RRID:Addgene_18354 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N53A N55A V57A E128A V131A N132A | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme C54S C97A Resource Report Resource Website 1+ mentions |
RRID:Addgene_18355 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C54S C97A | 2026-08-15 01:11:34 | 1 | |
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T4 Lysozyme N55G WT* Resource Report Resource Website |
RRID:Addgene_18356 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N55G WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme G56M WT* Resource Report Resource Website |
RRID:Addgene_18357 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | G56M WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme I58A WT* Resource Report Resource Website |
RRID:Addgene_18359 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | I58A WT* | 2026-08-15 01:11:34 | 0 | |
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pRET.IS.IRES-EGFP N2 Resource Report Resource Website |
RRID:Addgene_1834 | Ampicillin | PMID:10572187 | See scanned map. This is the original RET-EGFP vector. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. There are two versions, N1 and N2. Version N1 contains two XbaI sites and can be used for transfection after isolation of the XbaI-XbaI fragment. However, N1 contains an additional cryptic poly A signal between EGFP and GHpA, and its orientation is the same as that of virus transcription (from right to left in the figure). This means that N1 can not be used for the virus production: a full-length virus RNA is not produced efficiently. In contrast, N2 does not have such a cryptic poly A signal and is suitable for virus production. However, N2 contains an additional XbaI site between EGFP and GHpA, making it very difficult to isolate all the essential components of the vector on a single XbaI-XbaI fragment. Because of a weak NEO promoter, the number of G418-resistant colonies obtained after transfection (N1) or infection (N2) is fewer than that generated by other RET vectors with a strong NEO promoter. (Leder #C-1011) | Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:34 | 0 | |||
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pJS647 Resource Report Resource Website |
RRID:Addgene_183176 | PYR1 | Arabidopsis thaliana | Kanamycin | PMID:35726092 | Backbone Size:1774; Vector Backbone:pYTK084; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:11:34 | 0 | ||
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pRET.IL.IRES-EGFP (No HSV-TK) Resource Report Resource Website |
RRID:Addgene_1836 | Ampicillin | PMID:10572187 | See scanned map (#4). The HSV-TK cassette is eliminated from this vector. Virus production should be fine, but you can not titrate the virus accurately because of the lack of the HSV-TK cassette, the only constitutive marker in RET vectors. You can easily excise all the essential components as an XbaI-XbaI fragment. To obtain larger numbers of G418-resistant clones, this vector carries a strong NEO promoter. This RET vector would be the first choice for making gene-disrupted mice by using transfected or infected ES cells. (Leder #C-1023) | Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:34 | 0 | |||
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pRET.IL.PGK-TK Resource Report Resource Website |
RRID:Addgene_1837 | Ampicillin | PMID:10572187 | See scanned map (#5). This is a modified RET vector exclusively for the DEL.BANK project (JC Schimenti, the Jackson Lab): the PGK promoter for the HSV-TK gene is much stronger than the MC1 promoter in other RET vectors. Virus production is virtually impossible. Either the PGK promoter or PGK poly A signal seems to be very inhibitory for the transcription of the virus genome. You can easily excise the essential components as an XbaI-XbaI fragment. (Leder #C-1024) | Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:34 | 0 | |||
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T4 Lysozyme S44-[AAAA] WT* Resource Report Resource Website |
RRID:Addgene_18320 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S44-[AAAA] WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme S44D WT* Resource Report Resource Website |
RRID:Addgene_18322 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S44D WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme S44E WT* Resource Report Resource Website |
RRID:Addgene_18323 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S44E WT* | 2026-08-15 01:11:34 | 0 | |
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pRET.IL (No IRES-EGFP) Resource Report Resource Website |
RRID:Addgene_1832 | Ampicillin | PMID:10572187 | See scanned map. This is the original RET construct for in vitro mutagenesis experiments. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. This construct can not be used for expresssion pattern analysis because of lack of IRES-EGFP. The XbaI-XbaI fragment can be used for transfection. Enhancer sequence in the U3 portion of the 5' LTR is not included in this XbaI-XbaI fragment. (Leder #C-1021) | Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:34 | 0 | |||
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T4 Lysozyme S44F WT* Resource Report Resource Website |
RRID:Addgene_18324 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S44F WT* | 2026-08-15 01:11:34 | 0 | |
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pRET.IS.IRES-EGFP N1 Resource Report Resource Website |
RRID:Addgene_1833 | Ampicillin | PMID:10572187 | See scanned map. This is the original RET-EGFP vector. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. There are two versions, N1 and N2. Version N1 contains two XbaI sites and can be used for transfection after isolation of the XbaI-XbaI fragment. However, N1 contains an additional cryptic poly A signal between EGFP and GHpA, and its orientation is the same as that of virus transcription (from right to left in the figure). This means that N1 can not be used for the virus production: a full-length virus RNA is not produced efficiently. In contrast, N2 does not have such a cryptic poly A signal and is suitable for virus production. However, N2 contains an additional XbaI site between EGFP and GHpA, making it very difficult to isolate all the essential components of the vector on a single XbaI-XbaI fragment. Because of a weak NEO promoter, the number of G418-resistant colonies obtained after transfection (N1) or infection (N2) is fewer than that generated by other RET vectors with a strong NEO promoter. (Leder #C-1010) | Backbone Size:0; Vector Backbone:pRET; Vector Types:Mammalian Expression, Retroviral, Cre/Lox; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:34 | 0 | |||
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T4 Lysozyme S44H WT* Resource Report Resource Website |
RRID:Addgene_18326 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S44H WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme S44K WT* Resource Report Resource Website |
RRID:Addgene_18327 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S44K WT* | 2026-08-15 01:11:34 | 0 | |
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T4 Lysozyme S44P WT* Resource Report Resource Website |
RRID:Addgene_18330 | T4 Lysozyme | Bacteriophage T4 | Ampicillin | The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. | Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S44P WT* | 2026-08-15 01:11:34 | 0 |
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