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Species: Synthetic
Genetic Insert: SpyCatcher-M2e
Vector Backbone Description: Backbone Size:5676; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33468139
Comments: Original publications:
SpyCatcher:
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. doi: 10.1073/pnas.1115485109. Epub 2012 Feb 24. PMID: 22366317; PMCID: PMC3311370.
Proper citation: RRID:Addgene_165990 Copy
Species: Homo sapiens
Genetic Insert: Tln1Head (1-405)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific, Carlsbad, CA; Vector Backbone:pTrcHis C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33046605
Proper citation: RRID:Addgene_166127 Copy
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:5099; Vector Backbone:pBI-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9521327
Comments: Expression vector pBI-GFP was constructed by insertion of the blunt-ended HindIII/NotI GFP fragment of phGFP-S65T (Clontech) into the EcoRV site of pBI (Clontech).
Proper citation: RRID:Addgene_16623 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA H1047R
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV2-Tag 2A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15016963
Comments: This plasmid has 5 additional mutations in PIK3CA compared to NP_006209.2: N114D, L177S, T322A, A400V, L404P. These mutations do not alter the function of the encoded proteins.
Proper citation: RRID:Addgene_16639 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pIRESpuro2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11581659
Comments: This is an improved version of the vector used in the publication.
Note that although the SnapGene software recognizes the fluorescent protein as EYFP, this variant does not have the mutations required to convert GFP to YFP, and so should function more similarly to GFP.
Proper citation: RRID:Addgene_16616 Copy
Species: Homo sapiens
Genetic Insert: MnSOD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pBI-EGFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: Contains full length MnSOD gene including the mitochondrial leader sequence.
Proper citation: RRID:Addgene_16612 Copy
Species: Homo sapiens
Genetic Insert: PPAR delta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11226285
Comments: See author's map for more information. PKO2: used to delete exon 4 of PPAR delta. 5' arm is 1.8 kb and 3' arm is 4.0 kb.
Proper citation: RRID:Addgene_16619 Copy
Species: Other
Genetic Insert: Ty tag fused to mNeon
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166237 Copy
Species: Homo sapiens
Genetic Insert: shRNA for human YAP
Vector Backbone Description: Vector Backbone:PLKO.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166485 Copy
Species: Homo sapiens
Genetic Insert: HAUSP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15118411
Comments: A fragment of human HAUSP cDNA including the entire ORF was cloned into pCI-neo at the NheI and XbaI sites. (Please note that the XbaI site is blocked by overlapping methylation). A sequence including the Flag tag was added to one of the primers so that the encoded protein contains a Flag tag at the N-terminus.
Proper citation: RRID:Addgene_16655 Copy
Species: Homo sapiens
Genetic Insert: Cyclin E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pBI-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14999283
Comments: A fragment of human cyclin E cDNA including the entire ORF was cloned into pBI-EGFP.
Proper citation: RRID:Addgene_16654 Copy
Species: Homo sapiens
Genetic Insert: CDC4 R465C
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14999283
Comments: A 1.5kb fragment of human CDC4 cDNA (nucleotides 374-1874), which encompasses the entire ORF, was cloned into pCMV-Myc.
Addgene's quality control sequence suggests there are some unexpected mutations in the gene. The effect these mutations have on function is unknown, but this is the plasmid used in the Vogelstein article, so it should behave as reported.
Proper citation: RRID:Addgene_16653 Copy
Vector Backbone Description: Backbone Size:6807; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD133.48, Ligation number L4663.
Proper citation: RRID:Addgene_1665 Copy
Species: Mus musculus
Genetic Insert: mouse synuclein (from V. Lee)
Vector Backbone Description: Backbone Marker:Virginia Lee; Vector Backbone:pRK172; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33497415
Comments: The original plasmid reference is:
"Selective imaging of internalized proteopathic a-synuclein seeds in primary neurons reveals mechanistic insight into transmission of synucleinopathies" JBC 2017, DOI 0.1074/jbc.M117.780296
Richard J. Karpowicz, Jr. , Conor M. Haney , Tiberiu S. Mihaila , Raizel M. Sandler , E. James Petersson, and Virginia M.-Y. Lee
Proper citation: RRID:Addgene_166671 Copy
Species: Mus musculus
Genetic Insert: BimL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimL" (Venus fused to the N-terminus of the BH3-only protein, BimL) in live cells. There is a 3 amino acid flexible linker between Venus and BimL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166734 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: tRNA promoter : sgRNA cassette
Vector Backbone Description: Backbone Size:4988; Vector Backbone:pLSB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33313420
Comments: The following acknowledgement must be included in all research publications and other outputs: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-NAT was created with the support of the Wellcome Trust [095021], [200885], [203149]”.
Proper citation: RRID:Addgene_166698 Copy
Species: Homo sapiens
Genetic Insert: Bik
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VBik" (Venus fused to the N-terminus of the BH3-only protein, Bik) in live cells. There is an 8 amino acid flexible linker between Venus and Bik proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166737 Copy
Species: Mus musculus
Genetic Insert: tBid
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VtBid" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. There is a 3 amino acid flexible linker between Venus and tBid proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166736 Copy
Species: Mus musculus
Genetic Insert: BimEL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimEL" (Venus fused to the N-terminus of the BH3-only protein, BimEL) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL proteins. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166735 Copy
Species: synthetic/engineered
Genetic Insert: muGFP
Vector Backbone Description: Backbone Size:5405; Vector Backbone:pAM-DCA; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29330459
Proper citation: RRID:Addgene_166727 Copy
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