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Species: Mus musculus
Genetic Insert: Acidic Mammalian Chitinase
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pTwist CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38884443
Comments: Please visit https://doi.org/10.1101/2023.06.03.542675 for bioRxiv preprint.
Proper citation: RRID:Addgene_200228 Copy
Species: Mus musculus
Genetic Insert: H-2D(b) (CD8 mut)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Mutation prevents binding of CD8 (Potter et al. (1989) Nature doi.org/10.1038/337073a0). For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.
Proper citation: RRID:Addgene_180453 Copy
Species: Mus musculus
Genetic Insert: Sox17FNV
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37607832
Proper citation: RRID:Addgene_206380 Copy
Species: Mus musculus
Genetic Insert: miniSOX
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37607832
Proper citation: RRID:Addgene_206381 Copy
Species: Mus musculus
Genetic Insert: pAAV hSyn iGluSnFR3 v857.NGR
Vector Backbone Description: Backbone Marker:VectorBuilder; Backbone Size:5020; Vector Backbone:pAAV hSyn; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37142767
Proper citation: RRID:Addgene_196227 Copy
Species: Mus musculus
Genetic Insert: pAAV hSyn FLEX iGluSnFR3 v857.NGR
Vector Backbone Description: Backbone Size:5020; Vector Backbone:pAAV hSyn FLEX; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37142767
Proper citation: RRID:Addgene_196226 Copy
Species: Mus musculus
Genetic Insert: Anti-Nav1.2 Na+ channel (Rat) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934187. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199396 Copy
Species: Mus musculus
Genetic Insert: Anti-PINK1 (Human) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934189. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199399 Copy
Species: Mus musculus
Genetic Insert: Sstr3
Vector Backbone Description: Backbone Size:9859; Vector Backbone:pEF1α-IRES-EGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_200444 Copy
Species: Mus musculus
Genetic Insert: Anti-GFAP (Human) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934200. Isotype: IgG2b. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199410 Copy
Species: Mus musculus
Genetic Insert: recombinant mouse scFv targetingGFP (Aequorea victoria)
Vector Backbone Description: Backbone Size:6011; Vector Backbone:pCDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant scFv derived from RRID: AB_2934219.
Proper citation: RRID:Addgene_199429 Copy
Species: Mus musculus
Genetic Insert: recombinant mouse scFv targetingKir2.1 K+ channel (Rat)
Vector Backbone Description: Backbone Size:6011; Vector Backbone:pCDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant scFv derived from RRID: AB_2934213.
Proper citation: RRID:Addgene_199423 Copy
Species: Mus musculus
Genetic Insert: recombinant mouse scFv targetingKv3.1b K+ channel (Rat)
Vector Backbone Description: Backbone Size:6011; Vector Backbone:pCDNA3.4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant scFv derived from RRID: AB_2934214.
Proper citation: RRID:Addgene_199424 Copy
Species: Mus musculus
Genetic Insert: Anti-RGS14 (Rat) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934199. Isotype: IgG2b. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199409 Copy
Species: Mus musculus
Genetic Insert: Anti-Kir2.1 K+ channel (Rat) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934198. Isotype: IgG2b. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199408 Copy
Species: Mus musculus
Genetic Insert: Anti-Slo1/BKAlpha maxi-K+ channel (Rat) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934196. Isotype: IgG2b. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199406 Copy
Species: Mus musculus
Genetic Insert: Anti-Kv2.1 K+ channel (Rat) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2b; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934194. Isotype: IgG2b. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199404 Copy
Species: Mus musculus
Genetic Insert: Anti-VAPA (Rat) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934191. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199401 Copy
Species: Mus musculus
Genetic Insert: Anti-Kv1.7 K+ channel (Rat) recombinant mouse monoclonal antibody
Vector Backbone Description: Backbone Marker:Gavin Wright, Sanger; Yves Durocher, NRCC; Backbone Size:5925; Vector Backbone:P1316-IgG2a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37758930
Comments: This is a recombinant antibody derived from RRID: AB_2934190. Isotype: IgG2a. A portion of this plasmid was derived from a plasmid, pTT3, which was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute.
Proper citation: RRID:Addgene_199400 Copy
Species: Mus musculus
Genetic Insert: mCD47
Vector Backbone Description: Vector Backbone:pCW; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38215755
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.27.546594v1 for bioRxiv preprint.
Plasmid has been found to exist as a dimer. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_205449 Copy
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