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Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: NSP7
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141261 Copy
Species: Homo sapiens
Genetic Insert: TAPBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34039964
Proper citation: RRID:Addgene_141308 Copy
Species: Homo sapiens
Genetic Insert: TAPBPR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10381; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34039964
Proper citation: RRID:Addgene_141309 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF7B
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141277 Copy
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:13299; Vector Backbone:pRATIO1212; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141311 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141273 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF6
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141275 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5585; Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141276 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu-1
Genetic Insert: ORF3B
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32763951
Proper citation: RRID:Addgene_141272 Copy
Species: Bacillus phage PBS2, Petromyzon marinus, Streptococcus pyogenes
Genetic Insert: nCDA1-xBE3
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32005820
Proper citation: RRID:Addgene_141318 Copy
Vector Backbone Description: Backbone Marker:Aashima Khosla, David Nelson (unpublished); Backbone Size:14000; Vector Backbone:pRATIO2214; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141314 Copy
Species: Homo sapiens
Genetic Insert: TARDBP-A315T
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32325033
Proper citation: RRID:Addgene_141324 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10844; Vector Backbone:pGWB401; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141285 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141286 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141287 Copy
Species: Ruminococcus flavefaciens XPD3002
Genetic Insert: Rfx-Cas13d
Vector Backbone Description: Backbone Size:3150; Vector Backbone:pT3TS; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32768421
Comments: This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain) and Bazzini Lab (Stowers Institute, Kansas, MO, USA)
Proper citation: RRID:Addgene_141320 Copy
Species: Homo sapiens
Genetic Insert: TARDBP-G368A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32325033
Proper citation: RRID:Addgene_141325 Copy
Species: Homo sapiens
Genetic Insert: TARDBP-WT
Vector Backbone Description: Backbone Marker:Addgene# 24588; Backbone Size:9389; Vector Backbone:pLD-puro-Cc-VA (MAPLE vector); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32325033
Proper citation: RRID:Addgene_141327 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141299 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141296 Copy
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