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Species: Synthetic
Genetic Insert: human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
Vector Backbone Description: Backbone Marker:Addgene Plasmid #112101; Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38057426
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.20.524978v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185915 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Addgene Plasmid #112101; Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185914 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
Vector Backbone Description: Backbone Marker:Addgene Plasmid #112101; Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185913 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABE8e-nSpG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Addgene Plasmid #112101; Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38057426
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.20.524978v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185911 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
Vector Backbone Description: Backbone Marker:Addgene Plasmid #112101; Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185918 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABE8.20m-nSpRY-P2A-EGFP
Vector Backbone Description: Backbone Marker:Addgene Plasmid #112101; Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38057426
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.20.524978v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185917 Copy
Species: Synthetic
Genetic Insert: AGIA-AirID
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32391793
Proper citation: RRID:Addgene_182210 Copy
Species: Synthetic
Genetic Insert: TagBFP2
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118484 Copy
Species: Synthetic
Genetic Insert: sGFP2
Vector Backbone Description: Vector Backbone:pAG408; Vector Types:Bacterial Expression, miniTn5 delivery vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118526 Copy
Species: Synthetic
Genetic Insert: HygR2
Vector Backbone Description: Backbone Size:6372; Vector Backbone:pCk1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34076270
Proper citation: RRID:Addgene_185752 Copy
Species: Synthetic
Genetic Insert: HygR2
Vector Backbone Description: Backbone Size:6702; Vector Backbone:pCsA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34076270
Proper citation: RRID:Addgene_185751 Copy
Species: Synthetic
Genetic Insert: HygR2
Vector Backbone Description: Backbone Size:6702; Vector Backbone:CsA; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34076270
Proper citation: RRID:Addgene_185750 Copy
Species: Synthetic
Genetic Insert: PHAC1>Zif268>MED15>PABF1
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173283
Proper citation: RRID:Addgene_185839 Copy
Species: Synthetic
Genetic Insert: PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173283
Proper citation: RRID:Addgene_185842 Copy
Species: Synthetic
Genetic Insert: GFP-Y66-editing-site
Vector Backbone Description: Backbone Marker:Ramanujan Hegde; Backbone Size:5964; Vector Backbone:pmGFP-P2A-K0-P2A-RFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35232966
Comments: The reporter can be used in combination with pegRNA-GFP>BFP_Y66H-NGG with any type of prime editor. This pegRNA does not edit the human genome, therefore the combination of this reporter and the corresponding pegRNA can be cotransfected with your own pegRNA of interest to enrich for prime edited cells. Editing on the reporter will result in mCherry fluorescence.
Proper citation: RRID:Addgene_185477 Copy
Species: Synthetic
Genetic Insert: phiLOV3
Vector Backbone Description: Backbone Size:12; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34913537
Proper citation: RRID:Addgene_178973 Copy
Species: Synthetic
Genetic Insert: Lex-ER-B112
Vector Backbone Description: Backbone Marker:Voth et al., 2001; Vector Backbone:HO-Poly-KanMX4-HO; Vector Types:For yeast genomic integration; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35895499
Comments: Digest with PvuII for obtaining linear fragment for genomic integration to yeast into site 20 (Flagfeldt et al., 2009) via homologous recombination.
Proper citation: RRID:Addgene_183150 Copy
Species: Synthetic
Genetic Insert: LifeAct-jGCaMP8m
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_186039 Copy
Species: Synthetic
Genetic Insert: NES-jGCaMP8s
Vector Backbone Description: Vector Backbone:AAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_186037 Copy
Species: Synthetic
Genetic Insert: NES-jGCaMP8f
Vector Backbone Description: Vector Backbone:AAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_186035 Copy
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