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Genetic Insert: FRT-kan-FRT
Vector Backbone Description: Backbone Marker:M. Koob (University of Wisconsin, Madison); Vector Backbone:pANTSγ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:10829079
Comments: This is a template plasmids for frt-flanked kan cassette. The kan cassette originally came from pCP15.
pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.
Proper citation: RRID:Addgene_45605 Copy
Vector Backbone Description: Backbone Marker:Stratagene (Agilent Tech); Backbone Size:2864; Vector Backbone:pBluescript KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35416085
Proper citation: RRID:Addgene_187879 Copy
Species: Synthetic
Genetic Insert: Actb HR donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Proper citation: RRID:Addgene_97317 Copy
Species: Synthetic
Genetic Insert: Actb HMEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Comments: gRNA sequence: agtccgcctagaagcacttg
Proper citation: RRID:Addgene_97318 Copy
Species: Synthetic
Genetic Insert: Dbh HMEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Comments: gRNA sequence: agaatagcttctcacaaggt
Proper citation: RRID:Addgene_97320 Copy
Species: Schmidtea mediterranea
Genetic Insert: CRELD in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-AAAAATTGTGCCCATCTTCG, R-TGATTCCATCCTTCAGCACA
Proper citation: RRID:Addgene_99092 Copy
Species: Schmidtea mediterranea
Genetic Insert: sFRP1 (secreted frizzled related protein 1) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-AAACACTCAATGCCATGTCG, R-GTTGTCGCTGTCGATTTGTG
Proper citation: RRID:Addgene_99071 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smedwi-1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-CGTTCCTAAAGGCACAGCTC, R-TTGGATTAGCCCCATCTTTG
Proper citation: RRID:Addgene_99074 Copy
Species: Schmidtea mediterranea
Genetic Insert: Wnt1 (also known as WntP-1) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-AATCATCGCTGGAACTGTCC, R-TGTGGCAAATTTTGGTCGTA
Proper citation: RRID:Addgene_99073 Copy
Species: Schmidtea mediterranea
Genetic Insert: Activin (also known as activin 2) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-GGCCTTTGAAAGAACAGTGG, R-CTTCCTCGAGCGAAATTCAG
Proper citation: RRID:Addgene_99075 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smad2/3 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-CATGGAAACAAGCATTGTGG, R-GGTGGGATCTTGCAAACTGT
Proper citation: RRID:Addgene_99078 Copy
Species: Schmidtea mediterranea
Genetic Insert: Ras-related (also known as h.18.4a) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TGTGCGCTATCGTCAAAAAG, R-TTGAGATTACGAAGTCGACGAA
Proper citation: RRID:Addgene_99079 Copy
Species: Schmidtea mediterranea
Genetic Insert: Cyp1A1 (also known as h.49.4f) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TTGAAATTGGAGCGAAAATTAAA, R-GGGGAAATTCTTGAACCCTTC
Note: The Cyp1A1 insert contains several mismatches compared to AY067980.1. The depositor has confirmed this does not impact plasmid function.
Proper citation: RRID:Addgene_99081 Copy
Species: Other
Genetic Insert: eyes absent (eya)
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Proper citation: RRID:Addgene_99085 Copy
Species: Other
Genetic Insert: prohormone convertase 2 (PC2)
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TCGTTGCACACCTGTACCAT, R-CACTCCAACACCACAAATGC
Proper citation: RRID:Addgene_99087 Copy
Species: Schmidtea mediterranea
Genetic Insert: soxB (also known as soxB1-1) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TACTCATCCGCCCTTTCATC, R-CCGATCAATGTCTTCGGACT
Proper citation: RRID:Addgene_99086 Copy
Species: Schmidtea mediterranea
Genetic Insert: SoxB2-2 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-CGGTTACAGCGATGAAGACA, R-GAGAGTGACGGCAGAGTTCC
Proper citation: RRID:Addgene_99088 Copy
Species: Schmidtea mediterranea
Genetic Insert: Hesl-3 (hes-like) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-GGCAGAGCTCAAAGAATTGG, R-AACGGCCAGCTTTAGGATTT
Proper citation: RRID:Addgene_99090 Copy
Species: Schmidtea mediterranea
Genetic Insert: follistatin in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TATGTCCGGTCGGAGAGTTT, R-GAGGTGGGGCATTTGATACA
Proper citation: RRID:Addgene_99066 Copy
Species: Schmidtea mediterranea
Genetic Insert: LDLRR-2 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-GCCCAGACAAAGAAGATGGA, R-TGTTGCACAGCATTCGTTTT
Note: the LDLRR-2 insert has a 15 base pair deletion compared to JX010572.1. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_99094 Copy
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