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http://www.addgene.org/112900

Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:9412; Vector Backbone:pTORKm34GWR; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31575765
Comments: Please visit https://www.biorxiv.org/content/10.1101/652255v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_112900 Copy   

•   Source: Addgene

http://www.addgene.org/112903

Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:8431; Vector Backbone:pTORGm34GWC; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31775609

Proper citation: RRID:Addgene_112903 Copy   

•   Source: Addgene

http://www.addgene.org/112902

Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:6595; Vector Backbone:pTORGm34GW; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31775609

Proper citation: RRID:Addgene_112902 Copy   

•   Source: Addgene

http://www.addgene.org/112906

Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:8440; Vector Backbone:pTORGm34GWY; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31775609

Proper citation: RRID:Addgene_112906 Copy   

•   Source: Addgene

http://www.addgene.org/112905

Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:9151; Vector Backbone:pTORGm34GWR; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31775609

Proper citation: RRID:Addgene_112905 Copy   

•   Source: Addgene

http://www.addgene.org/112899

Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:8692; Vector Backbone:pTORKm34GWF; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31575765
Comments: Please visit https://www.biorxiv.org/content/10.1101/652255v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_112899 Copy   

•   Source: Addgene

http://www.addgene.org/113446

Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Luciferase, Gateway shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244

Proper citation: RRID:Addgene_113446 Copy   

•   Source: Addgene

http://www.addgene.org/113449

Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Gateway shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244

Proper citation: RRID:Addgene_113449 Copy   

•   Source: Addgene

http://www.addgene.org/113447

Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Luciferase, Gateway shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244

Proper citation: RRID:Addgene_113447 Copy   

•   Source: Addgene

http://www.addgene.org/11346

Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:see map of pACT2.2 in "author's map"; Backbone Size:7550; Vector Backbone:pACT2.2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified yeast two-hybrid Activation domain vector. Use this vector for Gateway cloning of a cDNA to be used to test a directed yeast two-hybrid interaction. This vector was modified from plasmid pACT2.2 by placing a Gateway acceptor cassette in the SmaI site of pACT2.2. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.) Please note that the "author's map" is the map of the original pACT2.2 vector upon which pACT2.2gtwy is derived - it is not a map of pACT2.2gtwy. This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Proper citation: RRID:Addgene_11346 Copy   

•   Source: Addgene

http://www.addgene.org/11345

Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:see map of pLexA in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified yeast two-hybrid DNA-binding domain vector. Use this vector for Gateway cloning of a cDNA to be used as the bait in a yeast two-hybrid screen. This vector was modified from plasmid pLexA by placing a Gateway acceptor cassette in the SmaI site of pLexA. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. Gateway recombinational cassettes should be added to primers as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation). Please note that the "author's map" is the map of the original pLexA vector upon which pLexAgtwy is derived - it is not a map of pLexAgtwy. This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Proper citation: RRID:Addgene_11345 Copy   

•   Source: Addgene

http://www.addgene.org/11344

Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified RNAi feeding vector L4440. Use this vector for Gateway cloning of an RNAi target sequence. A Gateway recombinational cassette was placed into the EcoRV site of vector L4440 to generate this modified plasmid. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.) This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

Proper citation: RRID:Addgene_11344 Copy   

•   Source: Addgene

http://www.addgene.org/113457

Vector Backbone Description: Backbone Marker:FUGW was a gift from David Baltimore (Addgene plasmid # 14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244

Proper citation: RRID:Addgene_113457 Copy   

•   Source: Addgene

http://www.addgene.org/114018

Genetic Insert: ccdB gene, Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin

Proper citation: RRID:Addgene_114018 Copy   

•   Source: Addgene

http://www.addgene.org/114017

Genetic Insert: ccdB gene, Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin

Proper citation: RRID:Addgene_114017 Copy   

•   Source: Addgene

http://www.addgene.org/15300

Vector Backbone Description: Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17574021
Comments: pWZL-Neo-Myr-Flag-DEST (pWN-MF-DEST) is a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF. Created by inserting an N-terminal myr-FLAG tag and the Gateway-selectable cassette into the pWzl-Neo retroviral vector. To create specific pWN-MF-ORF vectors, perform site-specific LR recombination reactions. LR reaction: 150ng DEST vector + 50ng ENTRY vector + 10xLR Buffer + LR enzyme + dH20. Incubate at 25oC for 1 hour. Add 1uL Proteinase K (20mg/ml), and incubate at 37oC for 10min. Transform into DH5alpha cells. See Invitrogen's website for detailed protocols and for LR enzyme.

Proper citation: RRID:Addgene_15300 Copy   

•   Source: Addgene

http://www.addgene.org/153243

Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_153243 Copy   

•   Source: Addgene

http://www.addgene.org/153241

Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_153241 Copy   

•   Source: Addgene

http://www.addgene.org/153246

Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_153246 Copy   

•   Source: Addgene

http://www.addgene.org/153245

Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33577114
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.

Proper citation: RRID:Addgene_153245 Copy   

•   Source: Addgene