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Species: Homo sapiens
Genetic Insert: XBP1
Vector Backbone Description: Vector Backbone:pShuttle2-Flag; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19416856
Proper citation: RRID:Addgene_63679 Copy
Species: Homo sapiens
Genetic Insert: Mini-intronic-plasmid intron
Vector Backbone Description: Backbone Size:7608; Vector Backbone:codon-optimized minicircle (CoMiC) construct; Vector Types:Mammalian Expression; Bacterial Resistance:RNA-OUT ( 6% sucrose)
Defining Citation: PMID:25628230
Comments: In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.
Proper citation: RRID:Addgene_63727 Copy
Species: Homo sapiens
Genetic Insert: Lin28
Vector Backbone Description: Vector Backbone:no backbone--uses MiP intron for replication in bacteria; Vector Types:Mammalian Expression; Bacterial Resistance:RNA-OUT ( 6% sucrose)
Defining Citation: PMID:25628230
Comments: In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.
Proper citation: RRID:Addgene_63729 Copy
Species: Homo sapiens
Genetic Insert: dCas9-VPR
Vector Backbone Description: Vector Backbone:PB-TRE; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology, Piggybac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Proper citation: RRID:Addgene_63800 Copy
Species: Homo sapiens
Genetic Insert: SP-dCas9-VPR
Vector Backbone Description: Vector Backbone:pcDNA3.3 TOPO; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 insert contains K604R, D839A and N863A mutations relative to reference sequence WP_010922251.1. Depositor states that these mutations should not affect function.
This plasmid contains a human codon optimized form of cas9.
Proper citation: RRID:Addgene_63798 Copy
Species: Homo sapiens
Genetic Insert: ST1-dCas9-VPR
Vector Backbone Description: Vector Backbone:pcDNA3.3 TOPO; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25730490
Comments: Cas9 insert contains K604R, D839A and N863A mutations relative to reference sequence WP_010922251.1. Depositor states that these mutations should not affect function.
Proper citation: RRID:Addgene_63799 Copy
Species: Homo sapiens
Genetic Insert: TT Fab
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pAra; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Provided with the pComb3H system for arabinose induced expression of soluble protein with detection tags. The “TT” refers to the human Fab to tetanus toxin which can be used as a protein expression control. Alternatively, one could upgrade to the pComb3X system, also available on Addgene, which already contains tags.
Select reference:
Cary, S.P.; Lee, J.; Wagenknecht, R.; Silverman, G.J. (2000) Characterization of Superantigen-Induced Clonal Deletion with a Novel Clan III-Restricted Avian Monoclonal Antibody: Exploiting Evolutionary Distance to Create Antibodies Specific for a Conserved VH Region Surface. J. Immun., 164, 4730-4741.
Proper citation: RRID:Addgene_63901 Copy
Species: Homo sapiens
Genetic Insert: AKT2
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25575350
Comments: The MYR sequence was inserted in frame at EcoRI/NheI site into N terminal of pLenti-FLAG-hAkt2
Please cite the following article:
Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).
Proper citation: RRID:Addgene_64050 Copy
Species: Homo sapiens
Genetic Insert: RAD21 Homology Arms
Vector Backbone Description: Backbone Size:5771; Vector Backbone:pFETCH_Donor; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid has been used along with PX548_RAD21_1 to add a FLAG tag to human RAD21 in HepG2 and MCF7 cells by the Mendenhall and Myers labs at HudsonAlpha Institute for Biotechnology/UAH. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_64056 Copy
Species: Homo sapiens
Genetic Insert: betaine-homocysteine methyltransferase
Vector Backbone Description: Backbone Marker:DNA 2.0; Vector Backbone:pJ414; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25884979
Proper citation: RRID:Addgene_64075 Copy
Species: Homo sapiens
Genetic Insert: mir-17-19b
Vector Backbone Description: Backbone Size:6216; Vector Backbone:pMSCV-SV40-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20008935
Proper citation: RRID:Addgene_64089 Copy
Species: Homo sapiens
Genetic Insert: miR-17-92
Vector Backbone Description: Backbone Size:6216; Vector Backbone:pMSCV-SV40-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24137534
Proper citation: RRID:Addgene_64094 Copy
Species: Homo sapiens
Genetic Insert: miR-17-92
Vector Backbone Description: Backbone Size:6216; Vector Backbone:pMSCV-SV40-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20008935
Proper citation: RRID:Addgene_64090 Copy
Species: Homo sapiens
Genetic Insert: hTRF1
Vector Backbone Description: Backbone Marker:de Lange Lab (Addgene plasmid# 12540); Vector Backbone:pLPC-N MYC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25344324
Proper citation: RRID:Addgene_64164 Copy
Species: Homo sapiens
Genetic Insert: miR-17-92
Vector Backbone Description: Backbone Size:7795; Vector Backbone:MSCV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24137534
Proper citation: RRID:Addgene_64100 Copy
Species: Homo sapiens
Genetic Insert: GFP-2A-BCL2L1
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25742692
Proper citation: RRID:Addgene_64123 Copy
Species: Homo sapiens
Genetic Insert: miR-17-92
Vector Backbone Description: Backbone Size:6216; Vector Backbone:pMSCV-SV40-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24137534
Proper citation: RRID:Addgene_64095 Copy
Species: Homo sapiens
Genetic Insert: sgRNA1_MYOD1
Vector Backbone Description: Backbone Marker:Stanley Qi, Addgene plasmid 44248; Vector Backbone:pgRNA-humanized; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25619936
Proper citation: RRID:Addgene_64137 Copy
Species: Homo sapiens
Genetic Insert: Pten 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20008935
Comments: Addgene's quality control sequence with Luc-F primer shows a single bp mismatch with the available NCBI reference sequence.
Proper citation: RRID:Addgene_64098 Copy
Species: Homo sapiens
Genetic Insert: sgRNA1_ASCL3
Vector Backbone Description: Backbone Marker:Stanley Qi, Addgene plasmid 44248; Vector Backbone:pgRNA-humanized; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25619936
Proper citation: RRID:Addgene_64131 Copy
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