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Species: Synthetic
Genetic Insert: EGFP-2A-Tetanus Toxin
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33704065
Proper citation: RRID:Addgene_166603 Copy
Genetic Insert: mTn7::MCS
Vector Backbone Description: Backbone Size:12549; Vector Backbone:pGRG36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16646962
Comments: Grow at 32'C. Same as pGRG25 except for the MCS. See author's protocol for more information.
This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.
Proper citation: RRID:Addgene_16666 Copy
Genetic Insert: mTn7::MCS
Vector Backbone Description: Backbone Size:12546; Vector Backbone:pGRG25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16646962
Comments: Grow at 32'C. Same as pGRG36 except for the MCS. See author's protocol for more information.
This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.
Proper citation: RRID:Addgene_16665 Copy
Species: Homo sapiens
Genetic Insert: BimEL(Mus musculus)-(Noxa BH3)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: BH3 region removed and inserted BH3 of Noxa protein. There is a K107E mutation in the Venus sequence of this plasmid. The K107E mutation is facing outwards in the B-Barrel structure of the Venus protein. Therefore, it is unlikely that this mutation would affect the chromophore within the β-barrel. We have transfected this plasmid in BMK-DKO cells and did not observe aggregation. We also used this construct and observed Forster resonance energy transfer from mCerulean3 in the context of measuring interactions with two binding partners. Thus, this mutation does not appear to affect the Venus fluorophore.
Proper citation: RRID:Addgene_166761 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6D; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33731932
Comments: Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.
Proper citation: RRID:Addgene_166835 Copy
Species: Lachnospiraceae bacterium C6A11
Genetic Insert: LahT150
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:5420; Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30638446
Comments: Please note: This plasmid exists as a multimer. This is not expected to impact the end function of the plasmid. Please refer to the Addgene Blog post on multimers for more information: https://blog.addgene.org/plasmids-101-dimers-and-multimers
Proper citation: RRID:Addgene_166785 Copy
Genetic Insert: Spike receptor binding domain (RBD) from SARS-CoV-2
Vector Backbone Description: Backbone Marker:Andrew Scharenberg; Vector Backbone:pETcon(-) (Plasmid #41522); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32841599
Proper citation: RRID:Addgene_166782 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: Gateway pDONR vector containing NLRP1 (no stop codon)
Proper citation: RRID:Addgene_166823 Copy
Species: Homo sapiens
Genetic Insert: Puma
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VPuma" (Venus fused to the N-terminus of the BH3-only protein, Puma) in live cells. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric. In cloning, the last amino acid of Venus protein was deleted (K239S). Result is a 1 amino acid linker between Venus and Puma proteins. The encoded BBC3 isoform 4 is also known as PUMA-alpha.
Proper citation: RRID:Addgene_166739 Copy
Species: Homo sapiens
Genetic Insert: Mcl-1
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 2nd generation lentiviral transfer plasmid that can be used to create lentivirus, for CMV driven expression of mCerulean3 fused to the N-terminus of Mcl-1. There is a flexible, 17 amino acid linker between mCerulean3 and Mcl-1. proteins.
Proper citation: RRID:Addgene_166752 Copy
Species: Mus musculus
Genetic Insert: Bcl-W
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: 3rd generation lentiviral transfer plasmid that can be used to create lentivirus, for hUbC-driven gene expression of mCerulean3-fused to the N-terminus of Bcl-W. There is a 7 aa linker between mCerulean3 and Bcl-W.
Proper citation: RRID:Addgene_166751 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35442739
Comments: Plasmid can be used to express monomeric Venus fluorophore in bacteria. We used E.coli BL21-AI (Arabinose induction) for protein production, induced 5h at 30oC. Purify protein via Nickel column chromatography. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166756 Copy
Species: Homo sapiens
Genetic Insert: Bad-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBad-4E" (Venus fused to the N-terminus of the BH3-only protein, Bad-4E) in live cells. There is a flexible, 7 amino acid linker between Venus and Bad-4E proteins. Full length Bad protein with BH3 region hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: Y110E, L114E, M117E, F122E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166740 Copy
Species: Bordetella pertussis
Genetic Insert: Pertussis toxin A protomer
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9389482
Comments: Insert: Linearize with Not1 and transcribe with SP6.
Ref: Slusarski et al., Nature 390:410-413, 1997.
Proper citation: RRID:Addgene_16678 Copy
Species: Mus musculus
Genetic Insert: tBid-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35442739
Comments: Transfection of this plasmid will express "VtBid-4E" (Venus fused to the N-terminus of the BH3-only protein, truncated Bid (tBid)) in live cells. In cells, caspase 8 cleavage of Bid produces the p15 fragment, referred to as tBid. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E). BH3, Mutations: I27E, L31E, I34E, M38E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166743 Copy
Species: Mus musculus
Genetic Insert: BimEL-4E
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express "VBimEL-4E" (Venus fused to the N-terminus of the BH3-only protein, BimEL-4E) in live cells. There is a 3 amino acid flexible linker between Venus and BimEL-4E proteins. BH3 region has hydrophobic positions H1-H4 mutated to glutamic acid (E), refered to as "BH3-4E" mutation, BH3 mutations: I146E, L150E, I153E, F157E. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_166742 Copy
Species: Homo sapiens
Genetic Insert: HRas 12V 37G
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Proper citation: RRID:Addgene_12589 Copy
Species: Homo sapiens
Genetic Insert: BAP1 (BRCA1 associated protein 1)
Vector Backbone Description: Backbone Marker:Feng Zhang; Vector Backbone:LentiCRISPR V2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30202049
Comments: Also see https://www.ncbi.nlm.nih.gov/pubmed/30907299.
Proper citation: RRID:Addgene_125837 Copy
Species: Bos taurus
Genetic Insert: p110-CAAX
Vector Backbone Description: Backbone Size:5558; Vector Backbone:pBabe hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Comments: Myc tagged p110-CAAX was cut out of pBabe-puro p110-CAAX using BamHI and ligated into BamHI cut pBabe-hygro.
Proper citation: RRID:Addgene_12591 Copy
Species: Homo sapiens
Genetic Insert: HRas 12V 40C
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11463831
Proper citation: RRID:Addgene_12590 Copy
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