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Species: Synthetic
Genetic Insert: [35S:pEPOR1CB0012(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117611 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117610 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-xCas9 3.7(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117615 Copy
Species: Synthetic
Genetic Insert: MAPPER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4758; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24183667
Proper citation: RRID:Addgene_117721 Copy
Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:2539; Vector Backbone:pIRESpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: codon-optimized A. thaliana AID (IAA17) is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117720 Copy
Species: Synthetic
Genetic Insert: [35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117605 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117609 Copy
Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4085; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: The codon-optimized A. thaliana AID (IAA17) is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117718 Copy
Species: Synthetic
Genetic Insert: mVenus-mAID
Vector Backbone Description: Backbone Size:5482; Vector Backbone:pIREShygro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: codon-optimized A. thaliana AID (IAA17) is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117714 Copy
Species: Synthetic
Genetic Insert: mAID-nanobody
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:4850; Vector Backbone:pCDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30120238
Comments: codon-optimized A. thaliana AID (IAA17) is described in:
Baker, O. et al. RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Sci Rep 6, 25529 (2016).
Proper citation: RRID:Addgene_117713 Copy
Species: Synthetic
Genetic Insert: sfCherry2(1-10)
Vector Backbone Description: Backbone Size:5420; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31552297
Comments: Please visit https://www.biorxiv.org/content/10.1101/454041v1 for BioRxiv preprint
Proper citation: RRID:Addgene_117656 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_145025 Copy
Species: Synthetic
Genetic Insert: ZsGreen1
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_192661 Copy
Species: Synthetic
Genetic Insert: TdTomato
Vector Backbone Description: Vector Backbone:pCR-Blunt II; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35763517
Proper citation: RRID:Addgene_192658 Copy
Species: Synthetic
Genetic Insert: Nluc
Vector Backbone Description: Vector Backbone:pCR-Blunt II; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35763517
Proper citation: RRID:Addgene_192657 Copy
Species: Synthetic
Genetic Insert: scFv-VP64
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35763517
Proper citation: RRID:Addgene_192655 Copy
Species: Synthetic
Genetic Insert: Nluc
Vector Backbone Description: Vector Backbone:pCR-Blunt II; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35763517
Proper citation: RRID:Addgene_192659 Copy
Species: Synthetic
Genetic Insert: Cas9, ZsGreen targeting sgRNA #4
Vector Backbone Description: Vector Backbone:LentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_192688 Copy
Species: Synthetic
Genetic Insert: 3JB1R_AP3+4bp (A Dual-Specificity aptamer targeting OGT and GFP)
Vector Backbone Description: Vector Backbone:pSilencer2.1-U6 hygro; Vector Types:Mammalian Expression, Synthetic Biology, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36626902
Comments: Sequence of the AP3 portion is from the article below:
Shui, B., Ozer, A., Zipfel, W., Sahu, N., Singh, A., Lis, J.T., Shi, H., and Kotlikoff, M.I. (2012). RNA aptamers that functionally interact with green fluorescent protein and its derivatives. Nucleic Acids Res 40, e39. 10.1093/nar/gkr1264.
Proper citation: RRID:Addgene_190841 Copy
Species: Synthetic
Genetic Insert: 4JC1FF (A Dual-Specificity aptamer targeting OGT and GFP)
Vector Backbone Description: Vector Backbone:pSilencer2.1-U6 hygro; Vector Types:Mammalian Expression, Synthetic Biology, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36626902
Comments: Sequence of the AP3 portion is from the article below:
Shui, B., Ozer, A., Zipfel, W., Sahu, N., Singh, A., Lis, J.T., Shi, H., and Kotlikoff, M.I. (2012). RNA aptamers that functionally interact with green fluorescent protein and its derivatives. Nucleic Acids Res 40, e39. 10.1093/nar/gkr1264.
Proper citation: RRID:Addgene_190846 Copy
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