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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pENTR-sfi_OsU3p_gRNA_SpecR_OsU3Ter Resource Report Resource Website |
RRID:Addgene_250279 | None | Spectinomycin | Backbone Size:2800; Vector Backbone:pENTR233-SfiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin | 2026-08-29 01:26:21 | 0 | ||||
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pENTR223-FDX1 Resource Report Resource Website |
RRID:Addgene_251696 | FDX1 ferredoxin 1 | Homo sapiens | Spectinomycin | PMID:40215978 | Backbone Size:2815; Vector Backbone:pENTR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | 2026-08-29 01:26:21 | 0 | ||
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pCA0.325 Resource Report Resource Website |
RRID:Addgene_200622 | Spectinomycin | Operon building system compatible with the CyanoGate Kit. McCormick Lab also uses TOP10 cells for transformation. | Vector Backbone:pL0-SC; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-29 01:26:22 | 0 | ||||
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pC0.340 Resource Report Resource Website |
RRID:Addgene_200628 | RBS* | Synthetic | Spectinomycin | McCormick Lab also uses TOP10 cells for transformation. | Vector Backbone:pL0-SC; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-29 01:26:22 | 0 | ||
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pC0.341 Resource Report Resource Website |
RRID:Addgene_200629 | RBS_B0034 | Synthetic | Spectinomycin | McCormick Lab also uses TOP10 cells for transformation. | Vector Backbone:pL0-SC; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-29 01:26:22 | 0 | ||
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pICSL60020 Resource Report Resource Website |
RRID:Addgene_245671 | Barley Oleosin terminator | Hordeum vulgare | Spectinomycin | Level 0 terminator module used for GoldenGate assemblies using the MoClo overhang syntax | Backbone Size:2249; Vector Backbone:pICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BsaI/BpiI sites removed by point-mutation | 2026-08-29 01:26:23 | 0 | |
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pICSL30055 Resource Report Resource Website |
RRID:Addgene_245558 | N-Terminal tag 3xGly4Ser-VP16-2xNLS | Synthetic | Spectinomycin | Level 0 N-Terminal tag module used for GoldenGate assemblies using the MoClo overhang syntax | Backbone Size:2246; Vector Backbone:pICSL01002; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin | BsaI/BpiI sites removed by point-mutation | 2026-08-29 01:26:23 | 0 | |
|
pBTK1217 (pDS-Def1-HC) Resource Report Resource Website |
RRID:Addgene_251996 | Fragment of Defensin 1 gene | Apis mellifera | Spectinomycin | Please visit https://doi.org/10.64898/2026.01.18.700149 for bioRxiv preprint. | Vector Backbone:pUC; Vector Types:Insect Expression; Bacterial Resistance:Spectinomycin | 2026-08-29 01:26:25 | 0 | ||
|
pCS10 Resource Report Resource Website |
RRID:Addgene_253620 | FLP recombinase | Spectinomycin | Alternative name: pRC453 Usage/Comments: This plasmid is used to generate a cSAGE-compatible base strain. Once pSC01 is integrated into the chromosome, this plasmid expresses FLP recombinase to cure / excise the backbone marker and contains a SacB marker for sucrose counterselection. This plasmid is used only for installation of the landing pad and is not required for downstream integration steps after the base strain is constructed. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint. | Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-29 01:27:54 | 0 | |||
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BdCESA8::LUC Resource Report Resource Website |
RRID:Addgene_256446 | Luciferase | Firefly | Spectinomycin | PMID:40236254 | Please visit https://doi.org/10.1101/2025.04.03.647122 for bioRxiv preprint. | Vector Backbone:pIPKb001; Vector Types:Luciferase; Bacterial Resistance:Spectinomycin | 2026-08-29 01:27:54 | 0 | |
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BdPMT::LUC Resource Report Resource Website |
RRID:Addgene_256447 | Luciferase | Firefly | Spectinomycin | PMID:40236254 | Please visit https://doi.org/10.1101/2025.04.03.647122 for bioRxiv preprint. | Vector Backbone:pIPKb001; Vector Types:Luciferase; Bacterial Resistance:Spectinomycin | 2026-08-29 01:27:54 | 0 | |
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pSPIN-GG Resource Report Resource Website |
RRID:Addgene_251750 | Spectinomycin | PMID:42420722 | Backbone Size:13712; Vector Backbone:pSC101*; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-29 01:27:55 | 0 | ||||
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pDGB3omega1_[10x 2EBS-S10:35Smp:NLS:YPet:35Sterm]-[tNOS:BASTA:pNOS] Resource Report Resource Website |
RRID:Addgene_254064 | 10x 2EBS-S10:35Smp:NLS:YPet:35Sterm | Synthetic | Spectinomycin | PMID:38379432 | Vector Backbone:pDGB3omega1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin | 2026-08-29 01:27:56 | 0 | ||
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pART27::PtGFP Resource Report Resource Website |
RRID:Addgene_258579 | PtGFP | Ptilosarcus sp. CSG-2001 | Spectinomycin | PMID:16600023 | pART27::PtGFP is the binary vector harbouring the NotI-fragment subcloned from pART7::PtGFP. It is for stable expression of PtGFP in the cytoplasm of higher plants via agrobacterium mediated transfer of the tDNA into the plant genome (Clough and Bent 1998 PubMed: 10069079; DOI: 10.1007/BF00028910). Note: The vector confers Spectinomycin resistance to bacteria (E. coli and Agrobacterium tumefaciens), but confers Kanamycin resistance to plants. PtGFP (GenBank AY015995) is a green fluorescent protein from the sea pen Ptilosarcus gurneyi which has been expressed in plants and used as a ratiometric pH indicator (Schulte et al. 2006 doi.org/10.1186/1746-4811-2-7, PubMed ID: 16600023). PtGFP is more photostable and less sensitive to acidic environments when compared with pHluorins (GenBank AF058694; GenBank AF058695). | Backbone Marker:Andrew Gleave (Addgene #247986); Backbone Size:11612; Vector Backbone:pART27; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin | 2026-08-29 01:27:55 | 0 | |
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pDONR223_NKX2-1_V205F Resource Report Resource Website |
RRID:Addgene_221473 | NKX2-1 | Homo sapiens | Spectinomycin | PMID:37994369 | Cloning based on backbone of 371AA short isoform of NKX2-1 ORF | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | V205F | 2026-08-29 01:21:22 | 0 |
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pDONR223_NKX2-1_DelNTerm_160-371AA Resource Report Resource Website |
RRID:Addgene_221475 | NKX2-1 | Homo sapiens | Spectinomycin | PMID:37994369 | Cloning based on backbone of 371AA short isoform of NKX2-1 ORF | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | Deletion of 1-159AA N-terminal domain | 2026-08-29 01:21:19 | 0 |
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pDONR223_NKX2-1_W208L Resource Report Resource Website |
RRID:Addgene_221474 | NKX2-1 | Homo sapiens | Spectinomycin | PMID:37994369 | Cloning based on backbone of 371AA short isoform of NKX2-1 ORF | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | W208L | 2026-08-29 01:21:19 | 0 |
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pDONR223_NKX2-1_HD_160-223AA Resource Report Resource Website |
RRID:Addgene_221477 | NKX2-1 | Homo sapiens | Spectinomycin | PMID:37994369 | Cloning based on backbone of 371AA short isoform of NKX2-1 ORF | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | Deletion of 1-159AA N-terminal and 224-371AA C-terminal domains | 2026-08-29 01:21:19 | 0 |
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pDONR223_NKX2-1_DelCTerm_1-223AA Resource Report Resource Website |
RRID:Addgene_221476 | NKX2-1 | Homo sapiens | Spectinomycin | PMID:37994369 | Cloning based on backbone of 371AA short isoform of NKX2-1 ORF | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | Deletion of 224-371AA C-terminal domain | 2026-08-29 01:21:22 | 0 |
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pDONR223_GFP Resource Report Resource Website |
RRID:Addgene_221469 | EGFP | Synthetic | Spectinomycin | PMID:37994369 | Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin | 2026-08-29 01:21:19 | 0 |
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