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Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS416; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14195 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS416; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14196 Copy
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:31980824
Comments: λ- F- glnX44 e14- (McrA-) rfbD1 endA1 thi-1 Δ(yjiT-opgB)114::IS10 (EcoKI R- M- McrBC- Mrr-) + rpoS393(am) creC510 lrhA::IS3 ydeN::IS10 ΔlacI
Verification of lacI deletion:
PCR reaction on genomic DNA using AK362 and AK365 primers produces a 1936 bp fragment
AK 362 5’-CAATACCAATCGCACGCGG
AK 365 5’-CGAGACGTCACGGAAAATGCC
Phenotype: Constitutive β-galactosidase synthesis
This strain was derived from the precursor strain, E. coli ER1821, which is described in Jobling et al. (2016) Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems. Genome Announc 4(4):e00763-16. https://www.ncbi.nlm.nih.gov/pubmed/27516504
Proper citation: RRID:Addgene_141407 Copy
Species: Homo sapiens
Genetic Insert: SOX9
Vector Backbone Description: Vector Backbone:FUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30804533
Comments: FUW-tetO-GFP (Addgene 30130) was digested with EcoRI to remove the GFP fragment and SOX9 was inserted
Proper citation: RRID:Addgene_141404 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS304; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14207 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS426; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14203 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS414; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14168 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS425; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14201 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14169 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS424; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14200 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS306; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14163 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14161 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14170 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14209 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2-orf3a
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:8825; Vector Backbone:pLVX-EF1alpha-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353859
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.22.002386v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_141383 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS426; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14179 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS305; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14210 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS425; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14178 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS306; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: integrating.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14211 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS424; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14176 Copy
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