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Species: M. alvus
Genetic Insert: 3-nitrotyrosine RS (F5)
Vector Backbone Description: Backbone Size:3220; Vector Backbone:pAJE; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:42037767
Comments: 3-nitrotyrosine RS (F5) insert is the same as Plasmid #225684, the difference being the low-copy pAJE origin (B95 cell compatible).
Proper citation: RRID:Addgene_249487 Copy
Genetic Insert: FLP recombinase
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Comments: Alternative name: pRC453
Usage/Comments: This plasmid is used to generate a cSAGE-compatible base strain. Once pSC01 is integrated into the chromosome, this plasmid expresses FLP recombinase to cure / excise the backbone marker and contains a SacB marker for sucrose counterselection. This plasmid is used only for installation of the landing pad and is not required for downstream integration steps after the base strain is constructed. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.
Proper citation: RRID:Addgene_253620 Copy
Species: Firefly
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pIPKb001; Vector Types:Luciferase; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40236254
Comments: Please visit https://doi.org/10.1101/2025.04.03.647122 for bioRxiv preprint.
Proper citation: RRID:Addgene_256446 Copy
Species: Firefly
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pIPKb001; Vector Types:Luciferase; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40236254
Comments: Please visit https://doi.org/10.1101/2025.04.03.647122 for bioRxiv preprint.
Proper citation: RRID:Addgene_256447 Copy
Vector Backbone Description: Backbone Size:13712; Vector Backbone:pSC101*; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:42420722
Proper citation: RRID:Addgene_251750 Copy
Species: Synthetic
Genetic Insert: 10x 2EBS-S10:35Smp:NLS:YPet:35Sterm
Vector Backbone Description: Vector Backbone:pDGB3omega1; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38379432
Proper citation: RRID:Addgene_254064 Copy
Species: Ptilosarcus sp. CSG-2001
Genetic Insert: PtGFP
Vector Backbone Description: Backbone Marker:Andrew Gleave (Addgene #247986); Backbone Size:11612; Vector Backbone:pART27; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16600023
Comments: pART27::PtGFP is the binary vector harbouring the NotI-fragment subcloned from pART7::PtGFP. It is for stable expression of PtGFP in the cytoplasm of higher plants via agrobacterium mediated transfer of the tDNA into the plant genome (Clough and Bent 1998 PubMed: 10069079; DOI: 10.1007/BF00028910). Note: The vector confers Spectinomycin resistance to bacteria (E. coli and Agrobacterium tumefaciens), but confers Kanamycin resistance to plants. PtGFP (GenBank AY015995) is a green fluorescent protein from the sea pen Ptilosarcus gurneyi which has been expressed in plants and used as a ratiometric pH indicator (Schulte et al. 2006 doi.org/10.1186/1746-4811-2-7, PubMed ID: 16600023). PtGFP is more photostable and less sensitive to acidic environments when compared with pHluorins (GenBank AF058694; GenBank AF058695).
Proper citation: RRID:Addgene_258579 Copy
Species: Synthetic
Genetic Insert: 10x 2EBS-S10:35Smp:NLS:GUS:35Sterm
Vector Backbone Description: Vector Backbone:pDGB3omega2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38379432
Proper citation: RRID:Addgene_254066 Copy
Species: Synthetic
Genetic Insert: EBSn:35Smp:GUS-(ggggs)x2-1x YPet:35Sterm
Vector Backbone Description: Vector Backbone:pDGB3omega2; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40976865
Proper citation: RRID:Addgene_254069 Copy
Species: Arabidopsis thaliana
Genetic Insert: DM2h[Bla-1]
Vector Backbone Description: Vector Backbone:pZZ006; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_246382 Copy
Vector Backbone Description: Vector Backbone:PZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41967643
Proper citation: RRID:Addgene_254170 Copy
Vector Backbone Description: Vector Backbone:PZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41967643
Proper citation: RRID:Addgene_254178 Copy
Vector Backbone Description: Vector Backbone:PZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41967643
Proper citation: RRID:Addgene_254181 Copy
Vector Backbone Description: Vector Backbone:PZP200; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41967643
Proper citation: RRID:Addgene_254183 Copy
Species: Homo sapiens
Genetic Insert: FDX1 ferredoxin 1
Vector Backbone Description: Backbone Size:2815; Vector Backbone:pENTR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40215978
Proper citation: RRID:Addgene_251696 Copy
Vector Backbone Description: Vector Backbone:pL0-SC; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Operon building system compatible with the CyanoGate Kit. McCormick Lab also uses TOP10 cells for transformation.
Proper citation: RRID:Addgene_200622 Copy
Species: Synthetic
Genetic Insert: RBS*
Vector Backbone Description: Vector Backbone:pL0-SC; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: McCormick Lab also uses TOP10 cells for transformation.
Proper citation: RRID:Addgene_200628 Copy
Species: Synthetic
Genetic Insert: RBS_B0034
Vector Backbone Description: Vector Backbone:pL0-SC; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: McCormick Lab also uses TOP10 cells for transformation.
Proper citation: RRID:Addgene_200629 Copy
Species: Hordeum vulgare
Genetic Insert: Barley Oleosin terminator
Vector Backbone Description: Backbone Size:2249; Vector Backbone:pICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 terminator module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245671 Copy
Species: Synthetic
Genetic Insert: N-Terminal tag 3xGly4Ser-VP16-2xNLS
Vector Backbone Description: Backbone Size:2246; Vector Backbone:pICSL01002; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 N-Terminal tag module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245558 Copy
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