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Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Fragments of assembly factors , with T7 promoter and terminator, with type II restriction cut sites at both ends were ordered from Twist Bioscience, construct was formed with a golden gate pot reaction to an receiver pCDF backbone.
Proper citation: RRID:Addgene_229507 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Gene Synthesis from Genscript. Each assembly factor is regulated by a individual T7 promoter and terminator.
Proper citation: RRID:Addgene_229509 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Fragments of assembly factors , with T7 promoter and terminator, with type II restriction cut sites at both ends were ordered from Twist Bioscience, construct was formed with a golden gate pot reaction to an receiver pCDF backbone.
Proper citation: RRID:Addgene_229508 Copy
Vector Backbone Description: Backbone Marker:Jores et al, 2020; Backbone Size:5464; Vector Backbone:pPSup; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40485594
Proper citation: RRID:Addgene_226912 Copy
Species: Homo sapiens
Genetic Insert: AHDC1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-hSyn-EGFP; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41605211
Comments: See publication for corresponding AAV Link vectors.
Proper citation: RRID:Addgene_236673 Copy
Species: Homo sapiens
Genetic Insert: AHDC1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-hSyn-EGFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41605211
Comments: See publication for corresponding AAV Link vectors.
Proper citation: RRID:Addgene_236672 Copy
Species: Homo sapiens
Genetic Insert: KDM5C
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-hSyn-EGFP; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41605211
Comments: See publication for corresponding AAV Link vectors.
Proper citation: RRID:Addgene_236671 Copy
Species: Homo sapiens
Genetic Insert: KDM5C
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-hSyn-EGFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41605211
Comments: See publication for corresponding AAV Link vectors.
Proper citation: RRID:Addgene_236670 Copy
Genetic Insert: Halo-SspB-PPM1H
Vector Backbone Description: Vector Backbone:p3xFLAG-CMV10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40211074
Comments: Please visit https://doi.org/10.1101/2024.06.08.598070 for bioRxiv preprint.
Proper citation: RRID:Addgene_232870 Copy
Species: Homo sapiens
Genetic Insert: mCherry-OSBP
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40211074
Comments: Please visit https://doi.org/10.1101/2024.06.08.598070 for bioRxiv preprint.
Proper citation: RRID:Addgene_232871 Copy
Species: Mus musculus
Genetic Insert: Neurocan-GFP
Vector Backbone Description: Backbone Size:3721; Vector Backbone:pAAV-CMVie; Vector Types:AAV; Bacterial Resistance:Ampicillin
Comments: Dr. Smith cloned the gene and used it in zebrafish (De Angelis et al., 2017, Dev Cell). She sent us the plasmid back in 2018. We cloned it into an AAV vector, and added the WPRE sequence. We have also validated its use in mouse and rat, and checked that it labels hyaluronan without affecting its properties and metabolism (Fernandez-Ballester et al., 2025, bioRxiv).
Please visit https://doi.org/10.1101/2025.01.31.635882 for bioRxiv preprint.
Proper citation: RRID:Addgene_235983 Copy
Species: Homo sapiens
Genetic Insert: UBN2
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-hSyn-EGFP; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41605211
Comments: See publication for corresponding AAV Link vectors.
Proper citation: RRID:Addgene_236679 Copy
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40130802
Comments: The construct features an N-terminal His6 tag followed by a 3C protease cleavage site, a 3_ FLAG tag, and a SUMO tag. The C-terminus includes a Thrombin cleavage site followed by an Opsin tag. BsaI recognition sites flank mScarlet3 to enable easy visualization of successful cloning through loss of red color. 5' Cloning Site: BsaI (destroyed), 3' Cloning Site: BsaI (destroyed).
Proper citation: RRID:Addgene_234684 Copy
Species: Synthetic
Genetic Insert: pTEF1>mTagBFP2>FrFAST
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40927980
Comments: URA3(-162,-1)>KlURA3>tKlURA3-pTEF1>mTagBFP2>FrFAST>tPGK1-URA3(609,905)
Proper citation: RRID:Addgene_223839 Copy
Species: Synthetic
Genetic Insert: pTEF1>mScarletI3>
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40927980
Comments: URA3(-162,-1)>KlURA3>tKlURA3-pTEF1>mScarletI3>tPGK1-URA3(609,905)
Proper citation: RRID:Addgene_223837 Copy
Species: Homo sapiens
Genetic Insert: IL-1Ra
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39706287
Comments: Please visit https://doi.org/10.1101/2024.01.31.578281 for bioRxiv preprint.
Proper citation: RRID:Addgene_223549 Copy
Species: Notothylas orbicularis
Genetic Insert: Rubisco large subunit
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3829; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39491367
Comments: Design of Notothylas orbicularis Rubisco expression plasmid mirrors Anthoceros agrestis Rubisco expression plasmid, pAaRbcLS as shown in Oh et al 2024 (10.1016/j.molp.2024.10.013).
Proper citation: RRID:Addgene_229520 Copy
Vector Backbone Description: Vector Backbone:pMS06; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40130927
Proper citation: RRID:Addgene_230071 Copy
Species: Homo sapiens
Genetic Insert: YTHDC2
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-hSyn-EGFP; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41605211
Comments: See publication for corresponding AAV Link vectors.
Proper citation: RRID:Addgene_236697 Copy
Species: Homo sapiens
Genetic Insert: YTHDC2
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pAAV-hSyn-EGFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41605211
Comments: See publication for corresponding AAV Link vectors.
Proper citation: RRID:Addgene_236696 Copy
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