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Species: Synthetic
Genetic Insert: sesRNA for human FXOP2
Vector Backbone Description: Vector Backbone:pAAV-hSyn-ClipF-sesRNA-smFlag-tTA2-W3SL; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36198803
Proper citation: RRID:Addgene_192067 Copy
Species: Synthetic
Genetic Insert: synGR (Part 3)
Vector Backbone Description: Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194480 Copy
Species: Synthetic
Genetic Insert: NcCas9
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36435853
Proper citation: RRID:Addgene_194093 Copy
Species: Synthetic
Genetic Insert: TadA 8e-nNcCas9
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36435853
Proper citation: RRID:Addgene_194095 Copy
Species: Synthetic
Genetic Insert: synDHBR (Part 3)
Vector Backbone Description: Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194478 Copy
Species: Synthetic
Genetic Insert: spCas9
Vector Backbone Description: Backbone Marker:Brett Stringer, Addgene #98291; Vector Backbone:plentiCRISPRv2 hygro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36342298
Proper citation: RRID:Addgene_193977 Copy
Species: Synthetic
Genetic Insert: fuBFP
Vector Backbone Description: Backbone Size:2633; Vector Backbone:pK18; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: fuBFP is an open-source superfolding blue fluorescent protein with excitation maximum at 376 nm and emission maximum at 447 nm. Relative to the original fuGFP, the protein contains the Y66H mutation (amino acid numbering based on classical GFP).
Proper citation: RRID:Addgene_191832 Copy
Species: Synthetic
Genetic Insert: Nanoluc-P2A-copGFP
Vector Backbone Description: Backbone Marker:SBI; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_192886 Copy
Species: Synthetic
Genetic Insert: 3xHA
Vector Backbone Description: Vector Backbone:vHB66; Vector Types:Pandoravirus/mollivirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36702819
Proper citation: RRID:Addgene_193457 Copy
Species: Synthetic
Genetic Insert: fuYFP
Vector Backbone Description: Backbone Size:2633; Vector Backbone:pK18; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: fuYFP is an open-source superfolding yellow fluorescent protein with excitation maximum at 514 nm and emission maximum at 529 nm. Relative to the original fuGFP, the protein contains the S65G and T203Y mutations (amino acid numbering based on classical GFP).
Proper citation: RRID:Addgene_191834 Copy
Species: Synthetic
Genetic Insert: synER Cassette
Vector Backbone Description: Backbone Size:1870; Vector Backbone:pYKT095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194484 Copy
Species: Synthetic
Genetic Insert: synGR Cassette
Vector Backbone Description: Backbone Size:1870; Vector Backbone:pYKT095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194485 Copy
Species: Synthetic
Genetic Insert: Uniq-CasRx (Codon optimized)
Vector Backbone Description: Backbone Size:8131; Vector Backbone:piggyBac Opie2-dsRed; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38108518
Proper citation: RRID:Addgene_194001 Copy
Species: Synthetic
Genetic Insert: synMR Cassette
Vector Backbone Description: Backbone Size:1870; Vector Backbone:pYKT095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194486 Copy
Species: Synthetic
Genetic Insert: Uniq-CasRx
Vector Backbone Description: Backbone Size:8134; Vector Backbone:piggyBac Opie2-dsRed; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38108518
Proper citation: RRID:Addgene_191374 Copy
Species: Synthetic
Genetic Insert: synDHBR Cassette
Vector Backbone Description: Backbone Size:1870; Vector Backbone:pYKT095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194483 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:n/a; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_192879 Copy
Species: Synthetic
Genetic Insert: tCYC1 (Part 4)
Vector Backbone Description: Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194492 Copy
Species: Synthetic
Genetic Insert: iEscSnFR
Vector Backbone Description: Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36868853
Comments: For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648.
Proper citation: RRID:Addgene_182807 Copy
Species: Synthetic
Genetic Insert: iFluoxSnFR_ER
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36868853
Comments: For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648.
Proper citation: RRID:Addgene_182815 Copy
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