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Species: Synthetic
Genetic Insert: iFluoxSnFR_PM
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:3373; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36868853
Comments: For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648.
Proper citation: RRID:Addgene_182816 Copy
Species: Synthetic
Genetic Insert: Cerulean
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36178266
Proper citation: RRID:Addgene_194048 Copy
Species: Synthetic
Genetic Insert: GB_SynP (A2) G1ab.3
Vector Backbone Description: Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36044643
Proper citation: RRID:Addgene_193124 Copy
Species: Synthetic
Genetic Insert: VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36178266
Proper citation: RRID:Addgene_194050 Copy
Species: Synthetic
Genetic Insert: VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36178266
Proper citation: RRID:Addgene_194051 Copy
Species: Synthetic
Genetic Insert: BE3
Vector Backbone Description: Vector Backbone:Addgene plasmid #124229; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36669486
Comments: Please visit https://doi.org/10.1101/2022.03.29.486051 for bioRxiv preprint.
Proper citation: RRID:Addgene_174570 Copy
Species: Synthetic
Genetic Insert: sesRNA for mouse Ctip2
Vector Backbone Description: Vector Backbone:pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36198803
Proper citation: RRID:Addgene_192068 Copy
Species: Synthetic
Genetic Insert: PE2-P2A-tagRFP-P2A-BleoR
Vector Backbone Description: Backbone Marker:David Liu; Vector Backbone:pCMV-PE2 (#132775); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36646933
Proper citation: RRID:Addgene_192508 Copy
Species: Synthetic
Genetic Insert: mVenus
Vector Backbone Description: Vector Backbone:pGWT35S; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36178266
Proper citation: RRID:Addgene_194000 Copy
Species: Synthetic
Genetic Insert: 37-12 Op (Part 2)
Vector Backbone Description: Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194490 Copy
Species: Synthetic
Genetic Insert: Zif268 Op (Part 2)
Vector Backbone Description: Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194491 Copy
Species: Synthetic
Genetic Insert: iEscSnFR_PM
Vector Backbone Description: Backbone Marker:Penn Vector Core; Backbone Size:3373; Vector Backbone:pAAV9-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36868853
Comments: For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648.
Proper citation: RRID:Addgene_182819 Copy
Species: Synthetic
Genetic Insert: iEscSnFR_ER
Vector Backbone Description: Backbone Marker:Penn Vector Core; Backbone Size:5005; Vector Backbone:pAAV9-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36868853
Comments: For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648.
Proper citation: RRID:Addgene_182818 Copy
Species: Synthetic
Genetic Insert: iFluoxSnFR_cyto
Vector Backbone Description: Backbone Marker:modified from Invitrogen; Backbone Size:5005; Vector Backbone:pCMV(MinDis); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36868853
Comments: For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648.
Please note the promoter was difficult to assemble with NGS. Due to this only a partial NGS result has been posted. Depositor confirms plasmid is functional.
Proper citation: RRID:Addgene_182817 Copy
Species: Synthetic
Genetic Insert: LOV-Turbo
Vector Backbone Description: Backbone Size:5366; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37188954
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.03.09.531939v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_199671 Copy
Species: Synthetic
Genetic Insert: sgRNA2_8xCTS
Vector Backbone Description: Vector Backbone:P2-ECFP-pA (Addgene #26280); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200244 Copy
Species: Synthetic
Genetic Insert: sgRNA1_8xCTS
Vector Backbone Description: Vector Backbone:P2-ECFP-pA (Addgene #26280); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539738v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200243 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eC VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075114
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_201903 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_194970 Copy
Species: Synthetic
Genetic Insert: JEDI-1P-Kv
Vector Backbone Description: Backbone Size:4545; Vector Backbone:pAAV-hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37828037
Comments: Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint.
Proper citation: RRID:Addgene_202612 Copy
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