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Species: Synthetic
Genetic Insert: Periplasmic targeting sequence ssDsbA fused to mNeongreen
Vector Backbone Description: Backbone Marker:novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42599979
Proper citation: RRID:Addgene_245932 Copy
Species: Simiaoa sunii
Genetic Insert: DddA
Vector Backbone Description: Backbone Marker:LifescienceMarket, Cat. #PVT0105; Vector Backbone:pColADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41339527
Comments: Please see the protocol document linked above, in the Resource Information section.
Proper citation: RRID:Addgene_254736 Copy
Species: Homo sapiens
Genetic Insert: Ska1 LZ-LMTBD
Vector Backbone Description: Backbone Size:5288; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.1101/2025.07.06.663352 for bioRxiv preprint.
Proper citation: RRID:Addgene_253361 Copy
Species: Synthetic
Genetic Insert: Array of guide RNAs
Vector Backbone Description: Vector Backbone:pK335; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42477113
Proper citation: RRID:Addgene_253418 Copy
Species: Synthetic
Genetic Insert: LumiScarlet x3, AkaLuc-P2A-mTagBFP-P2A-BlastR
Vector Backbone Description: Vector Backbone:pPD1178 Integration Vector (PuroR-P2A-miRFP720); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40315428
Comments: Leonard Lab plasmid reference number: L3475
Proper citation: RRID:Addgene_253897 Copy
Species: Nocardia ninae
Genetic Insert: Dob3
Vector Backbone Description: Backbone Size:5302; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41639443
Comments: Please visit https://doi.org/10.1101/2025.05.24.655970 for bioRxiv preprint.
Proper citation: RRID:Addgene_251541 Copy
Species: Nocardia ninae
Genetic Insert: Dob2
Vector Backbone Description: Backbone Size:5302; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41639443
Comments: Please visit https://doi.org/10.1101/2025.05.24.655970 for bioRxiv preprint.
Proper citation: RRID:Addgene_251540 Copy
Species: Nocardia ninae
Genetic Insert: DobA
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41639443
Comments: Please visit https://doi.org/10.1101/2025.05.24.655970 for bioRxiv preprint.
Proper citation: RRID:Addgene_251533 Copy
Species: Nocardia ninae
Genetic Insert: DobM
Vector Backbone Description: Backbone Size:5302; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41639443
Comments: Please visit https://doi.org/10.1101/2025.05.24.655970 for bioRxiv preprint.
Proper citation: RRID:Addgene_251538 Copy
Species: Nocardia ninae
Genetic Insert: DobG
Vector Backbone Description: Backbone Size:5302; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41639443
Comments: Please visit https://doi.org/10.1101/2025.05.24.655970 for bioRxiv preprint.
Proper citation: RRID:Addgene_251537 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Srs2-C-terminus
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_247774 Copy
Vector Backbone Description: Vector Backbone:JD633; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2026.02.10.705172 for bioRxiv preprint.
The reference articles to construct this plasmid:
A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875
A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
Proper citation: RRID:Addgene_253103 Copy
Species: Triticum aestivum and Triticum turgidum
Genetic Insert: SpCas9-P2A-RUBY
Vector Backbone Description: Vector Backbone:JD633; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2026.02.10.705172 for bioRxiv preprint.
The reference articles to construct this plasmid:
A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875
A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
Proper citation: RRID:Addgene_253104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO family protein SMT3
Vector Backbone Description: Backbone Size:5228; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41927759
Comments: This plasmid contains a SUMO protein construct to serve as a control protein for binding experiments using the AH-SUMO platform, to verify non-interaction between the SUMO domain and liposomes of choice. It contains the SUMO1-98 region of the SMT3_yeast gene, followed by a Cysteine, a TEV site and a hexa-histidine site. On the N-terminal of the SUMO domain, it contains a region encoding for MGSGS, which is there to mimic the structure of a AH-SUMO platform that contains an N-terminal peptide of interest , serving as a better control.
Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.
Proper citation: RRID:Addgene_254139 Copy
Species: Acinetobacter baumannii
Genetic Insert: mltB
Vector Backbone Description: Vector Backbone:pETAb; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_253611 Copy
Species: Homo sapiens
Genetic Insert: CAD
Vector Backbone Description: Backbone Marker:This study; Vector Backbone:pLenti-EF1alpha-DEST-IRES-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253784 Copy
Species: Homo sapiens
Genetic Insert: CAD
Vector Backbone Description: Backbone Marker:This study; Vector Backbone:pLenti-EF1alpha-DEST-IRES-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253785 Copy
Species: Homo sapiens
Genetic Insert: CAD
Vector Backbone Description: Backbone Marker:This study; Vector Backbone:pLenti-EF1alpha-DEST-IRES-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253786 Copy
Species: Synthetic
Genetic Insert: HaloTag-TEV-transmembrane domain-mCherry
Vector Backbone Description: Vector Backbone:pBIN20; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42086881
Comments: Please see the following preprints as well as their final/published form:
Lowensohn, T. N.; Cody, W. B.; Tsai, C.; Vlahos, A. E.; Call, C. C.; Gao, X. J.; Sattely, E. S. An in Planta Single-Cell Screen to Accelerate Functional Genetics. bioRxiv February 19, 2026, p 2025.08.06.668818. https://doi.org/10.1101/2025.08.06.668818.
Vlahos, A. E.; Call, C. C.; Eckman, N.; Kadaba, S. E.; Guo, S.; Thurm, A. R.; Kang, J.; Appel, E. A.; Bintu, L.; Gao, X. J. A Complete Suite for Compact Programming of Protein Secretion. bioRxiv May 27, 2025, p 2023.10.04.560774. https://doi.org/10.1101/2023.10.04.560774.
Proper citation: RRID:Addgene_254126 Copy
Species: Other
Genetic Insert: ABR, lv0, Akan, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249985 Copy
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