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Species: Homo sapiens
Genetic Insert: Delta(Pro-MP)ADAM10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12743035
Comments: Mutagenesis creates EcoRI site 57bp downstream of Start ATG
Proper citation: RRID:Addgene_65107 Copy
Species: Synthetic
Genetic Insert: MS2
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMal-c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18570876
Comments: The following reference describes how to isolate RNA binding complexes using this construct:
Jurica and Moore, Methods. 2002 Nov;28(3):336-45. https://www.ncbi.nlm.nih.gov/pubmed/12431437
Proper citation: RRID:Addgene_65104 Copy
Genetic Insert: ABKAR biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25892241
Proper citation: RRID:Addgene_65068 Copy
Genetic Insert: RBD, mCerulean, mVenus, RhoC
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24224016
Proper citation: RRID:Addgene_65071 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Glutaredoxin-1
Vector Backbone Description: Vector Backbone:p415TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23242256
Proper citation: RRID:Addgene_65004 Copy
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pdCas9-Marraffini (pACYC184); Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25822415
Proper citation: RRID:Addgene_65006 Copy
Species: Homo sapiens
Genetic Insert: Glutaredoxin-1
Vector Backbone Description: Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22100409
Comments: Sequence discrepancies found during QC should not affect function
Proper citation: RRID:Addgene_65000 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65141 Copy
Species: Synthetic
Genetic Insert: GFP dropout
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 234r is used for the construction of dropout vectors. This plasmid encodes for a fluorescent dropout. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65154 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3001; Vector Backbone:pGEM5Z(+); Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19248754
Comments: To be used in conjunction with pGEM5Z(+)-EGFP (p111): www.addgene.org/65206. See corrigendum/erratum for original publication (https://doi.org/10.1016/j.ab.2009.10.024) before performing experiments with this plasmid.
Proper citation: RRID:Addgene_65207 Copy
Species: Synthetic
Genetic Insert: ConR1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65174 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822.
VP1 capsid ORF utilizes a non-canonical initiation codon (TTG codon) beginning at nt 6514.
Additional article references: Barbash et al. Gene Therapy 20: 274-282 (2013).
Proper citation: RRID:Addgene_65215 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6824.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6511.
Proper citation: RRID:Addgene_65214 Copy
Species: Homo sapiens
Genetic Insert: esr1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65211 Copy
Species: Synthetic
Genetic Insert: NourseothricinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65185 Copy
Species: Synthetic
Genetic Insert: KanamycinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65184 Copy
Species: Homo sapiens
Genetic Insert: HERCD533
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1346334
Comments: Insert codes for AA 1-653 (extracellular and transmembrane domains) Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region.
Proper citation: RRID:Addgene_65224 Copy
Species: Synthetic
Genetic Insert: ZeocinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65187 Copy
Species: Pseudomonas putida strain CBB5
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23654268
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3.
Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100).
N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See:
Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049.
For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC).
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_65220 Copy
Species: Homo sapiens
Genetic Insert: AXL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5900; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18339872
Proper citation: RRID:Addgene_65222 Copy
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