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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pcDNA3-Delta(Pro-MP)ADAM10-HA Resource Report Resource Website 1+ mentions |
RRID:Addgene_65107 | Delta(Pro-MP)ADAM10 | Homo sapiens | Ampicillin | PMID:12743035 | Mutagenesis creates EcoRI site 57bp downstream of Start ATG | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted AA 19-455 | 2026-09-01 09:59:25 | 1 |
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pMBP-MS2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65104 | MS2 | Synthetic | Ampicillin | PMID:18570876 | The following reference describes how to isolate RNA binding complexes using this construct: Jurica and Moore, Methods. 2002 Nov;28(3):336-45. https://www.ncbi.nlm.nih.gov/pubmed/12431437 | Backbone Marker:NEB; Vector Backbone:pMal-c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | double mutation (V75Q and A81G) that prevents oligomerization | 2026-09-01 09:59:25 | 8 |
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LAMP1-ABKAR Resource Report Resource Website 1+ mentions |
RRID:Addgene_65068 | ABKAR biosensor | Ampicillin | PMID:25892241 | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:59:24 | 1 | |||
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RhoC FLARE.sc mCer, mVenus - wt Resource Report Resource Website 1+ mentions |
RRID:Addgene_65071 | RBD, mCerulean, mVenus, RhoC | Ampicillin | PMID:24224016 | Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin | T153M in mVenus | 2026-09-01 09:59:24 | 1 | ||
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p415TEF cyto roGFP2-Grx1(yeast) Resource Report Resource Website 1+ mentions |
RRID:Addgene_65004 | Glutaredoxin-1 | Saccharomyces cerevisiae | Ampicillin | PMID:23242256 | Vector Backbone:p415TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-09-01 09:59:24 | 3 | ||
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pCRISPathBrick Resource Report Resource Website 1+ mentions |
RRID:Addgene_65006 | Chloramphenicol | PMID:25822415 | Backbone Size:9326; Vector Backbone:pdCas9-Marraffini (pACYC184); Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-09-01 09:59:24 | 7 | ||||
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pCaSpeR4 mito roGFP2-Grx1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65000 | Glutaredoxin-1 | Homo sapiens | Ampicillin | PMID:22100409 | Sequence discrepancies found during QC should not affect function | Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | mitochondrial targeting sequence | 2026-09-01 09:59:24 | 1 |
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pYTK034 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65141 | mRuby2 | Synthetic | Chloramphenicol | PMID:25871405 | Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-09-01 09:59:25 | 2 | |
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pYTK047 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65154 | GFP dropout | Synthetic | Chloramphenicol | PMID:25871405 | Type 234r is used for the construction of dropout vectors. This plasmid encodes for a fluorescent dropout. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-09-01 09:59:26 | 3 | |
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p189 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65207 | EGFP | Aequorea victoria | Ampicillin | PMID:19248754 | To be used in conjunction with pGEM5Z(+)-EGFP (p111): www.addgene.org/65206. See corrigendum/erratum for original publication (https://doi.org/10.1016/j.ab.2009.10.024) before performing experiments with this plasmid. | Backbone Marker:Promega; Backbone Size:3001; Vector Backbone:pGEM5Z(+); Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin | Premature stop codon added to pGEM5Z(+)-EGFP (TGG(58)-->TAG) | 2026-09-01 09:59:26 | 3 |
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pYTK067 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65174 | ConR1 | Synthetic | Chloramphenicol | PMID:25871405 | Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-09-01 09:59:26 | 2 | |
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pSR646 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65215 | modified AAV-2 rep78 | Other | Ampicillin | PMID:19532142 | Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822. VP1 capsid ORF utilizes a non-canonical initiation codon (TTG codon) beginning at nt 6514. Additional article references: Barbash et al. Gene Therapy 20: 274-282 (2013). | Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-01 09:59:27 | 1 | |
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pSR657 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65214 | modified AAV-2 rep78 | Other | Ampicillin | PMID:19532142 | Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6824. VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6511. | Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin | 2026-09-01 09:59:26 | 4 | |
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GFP-ESR1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65211 | esr1 | Homo sapiens | Kanamycin | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-01 09:59:26 | 1 | |||
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pYTK078 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65185 | NourseothricinR | Synthetic | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-09-01 09:59:26 | 1 | |
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pYTK077 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65184 | KanamycinR | Synthetic | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-09-01 09:59:26 | 2 | |
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pRK5RS-HERCD533 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65224 | HERCD533 | Homo sapiens | Ampicillin | PMID:1346334 | Insert codes for AA 1-653 (extracellular and transmembrane domains) Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region. | Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted AA 654-1186 | 2026-09-01 09:59:27 | 1 |
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pYTK080 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65187 | ZeocinR | Synthetic | Chloramphenicol | PMID:25871405 | Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. | Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-09-01 09:59:26 | 1 | |
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pDCAF3-IS5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65220 | ndmA | Pseudomonas putida strain CBB5 | Chloramphenicol and Kanamycin | PMID:23654268 | The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3. Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100). N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See: Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049. For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC). Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin | 2026-09-01 09:59:27 | 1 | |
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pLXSN-Axl Resource Report Resource Website 1+ mentions |
RRID:Addgene_65222 | AXL | Homo sapiens | Ampicillin | PMID:18339872 | Backbone Marker:Clontech; Backbone Size:5900; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-09-01 09:59:27 | 1 |
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