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Species: Synthetic
Genetic Insert: Full length TSWV M antigenome encoding SpCas9 in place of viral GP
Vector Backbone Description: Vector Backbone:pCB301-2μ-HDV; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36751129
Comments: Note: Plasmid contains a R822Q mutation in the 3xFlag-SV40NLS-Cas9 insert. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_196325 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pLVSIN-CMV_Pur; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40399693
Comments: https://benchling.com/s/seq-axx2imqJK8S5TKwg957v?m=slm-g8zVa6H19po3x7oJHpso
Please visit https://www.biorxiv.org/content/10.1101/2023.01.18.524633v1 for bioRxiv preprint. This plasmid is relatively unstable due to repetitive sequences and other genetic element cloned from retrovirus. Please be sure to use NEB stable cells and incubate at 30C.
Proper citation: RRID:Addgene_197137 Copy
Species: Synthetic
Genetic Insert: Full length TSWV M antigenome encoding LbCas12a in place of viral GP
Vector Backbone Description: Vector Backbone:pCB301-2μ-HDV; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36751129
Comments: Please note: Plasmid contains K191N, E695D, and M985I mutations in LbCas12a. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_196291 Copy
Species: Synthetic
Genetic Insert: HDR donor template
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199451 Copy
Species: Synthetic
Genetic Insert: TS2 sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199454 Copy
Species: Synthetic
Genetic Insert: HDR donor template
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199453 Copy
Species: Synthetic
Genetic Insert: stdMCP-PH
Vector Backbone Description: Vector Backbone:pCS2(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199461 Copy
Species: Synthetic
Genetic Insert: Zebrafish NarTag
Vector Backbone Description: Vector Backbone:pCS2(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199460 Copy
Species: Synthetic
Genetic Insert: pSP6_Kozak
Vector Backbone Description: Backbone Marker:John Dueber Lab, https://doi.org/10.1021/sb500366v; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37009624
Proper citation: RRID:Addgene_200151 Copy
Species: Synthetic
Genetic Insert: tT7
Vector Backbone Description: Backbone Marker:John Dueber Lab, https://doi.org/10.1021/sb500366v; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37009624
Proper citation: RRID:Addgene_200153 Copy
Species: Synthetic
Genetic Insert: CRISPR-Tag
Vector Backbone Description: Vector Backbone:pMV; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36453882
Proper citation: RRID:Addgene_199448 Copy
Species: Synthetic
Genetic Insert: leuYFP[CTT]-nLuc
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:6656; Vector Backbone:pAG306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37783489
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.03.19.533377v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_199757 Copy
Species: Synthetic
Genetic Insert: leuYFP[CTC]
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:6656; Vector Backbone:pAG306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37783489
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.03.19.533377v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_199758 Copy
Species: Synthetic
Genetic Insert: Nanoluc
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:6656; Vector Backbone:pAG306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37783489
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.03.19.533377v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_199755 Copy
Species: Synthetic
Genetic Insert: NanolucPEST
Vector Backbone Description: Backbone Size:5449; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35491906
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.03.19.533377v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_199764 Copy
Species: Synthetic
Genetic Insert: synYFP[TTG/AGA]-6xArg[CGA]-nLuc
Vector Backbone Description: Backbone Marker:Susan Lindquist; Backbone Size:6656; Vector Backbone:pAG306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37783489
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.03.19.533377v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_199760 Copy
Species: Synthetic
Genetic Insert: humanized denOsCas12f1-VPR
Vector Backbone Description: Vector Backbone:piggyBac-CBh; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37041195
Proper citation: RRID:Addgene_197031 Copy
Species: Synthetic
Genetic Insert: humanized miniCRISRPoff-v1
Vector Backbone Description: Vector Backbone:piggyBac-CBh; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37041195
Proper citation: RRID:Addgene_197030 Copy
Species: Synthetic
Genetic Insert: Int.8 Spacer
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK090; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_198865 Copy
Species: Synthetic
Genetic Insert: humanized ecDHFR-enOsCas12f1-ecDHFR
Vector Backbone Description: Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37041195
Proper citation: RRID:Addgene_197029 Copy
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