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Species: Synthetic
Genetic Insert: J23115_TSS
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119606 Copy
Genetic Insert: PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:7295; Vector Backbone:PDGB3omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29680684
Proper citation: RRID:Addgene_119712 Copy
Genetic Insert: L3S3P11 terminator
Vector Backbone Description: Vector Backbone:ColE2 plasmid; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30575748
Proper citation: RRID:Addgene_120899 Copy
Genetic Insert: pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
Vector Backbone Description: Vector Backbone:ColE2 plasmid; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30575748
Proper citation: RRID:Addgene_120900 Copy
Species: Synthetic
Genetic Insert: DsRed-Express2
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pDL278; Vector Types:E. coli streptococcal shuttle vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31101614
Proper citation: RRID:Addgene_121505 Copy
Vector Backbone Description: Backbone Size:4705; Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117234 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-DE (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117523 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-KA (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117524 Copy
Species: Streptococcus pyogenes
Genetic Insert: Sp-eCas9 1.0 (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117526 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:19837844
Proper citation: RRID:Addgene_117479 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pYAO:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117513 Copy
Species: Synthetic
Genetic Insert: BsaI:AATG:Cas9_4:GCTT:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117516 Copy
Species: Synthetic
Genetic Insert: BsaI:AATG:Cas9_3(intron):GCTT:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel
This plasmid is unstable in E. coli. After transformation, many positive colonies will actually contains a truncated version of the insert, generally around the intron. The plasmid should be carefully verified on gel after restriction enzyme digestion to make sure the all insert is present. Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117515 Copy
Species: Escherichia coli
Genetic Insert: Aph(3')-IIa promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21601101
Proper citation: RRID:Addgene_117481 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pEC1.2:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117507 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pUBI10:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Mark Youles . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117506 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pEC_enh:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlison . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117508 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117532 Copy
Species: Streptococcus pyogenes
Genetic Insert: Sp-xCas9 3.7 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117530 Copy
Species: Acidaminococcus sp. BV3L6
Genetic Insert: AsCas12a (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117535 Copy
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