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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide2 Resource Report Resource Website |
RRID:Addgene_118174 | KRAB-dCas9-2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function. | Backbone Marker:Jorge Ferrer; Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:55 | 0 | |
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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide3 Resource Report Resource Website |
RRID:Addgene_118171 | KRAB-dCas9-2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function. | Backbone Marker:Jorge Ferrer; Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:55 | 0 | |
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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2 Resource Report Resource Website |
RRID:Addgene_118170 | KRAB-dCas9-2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function. | Backbone Marker:Jorge Ferrer; Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:52 | 0 | |
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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR Resource Report Resource Website |
RRID:Addgene_118155 | KRAB-dCas9-P2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | We have also generated a version of this backbone with the core EF1a promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest. In our experience this backbone yields better knockdown in liver and pancreatic beta cells. Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. We have also made available plasmids to be used as negative controls, which contain non-human genome target sequences (BFP or EGFP). Please see Addgene #118161, #118162, #118163 and #118164. There is a third BsmBI site in the promoter region, which does not have an impact on the GoldenGate cloning. We recommend using the provided cloning protocol. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function. | Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:55 | 0 | |
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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR Resource Report Resource Website 1+ mentions |
RRID:Addgene_118154 | KRAB-dCas9-P2A-BlastR | Homo sapiens | Ampicillin | We have also generated a version of this backbone with the hPGK promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function. | Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | See depositor comments below | 2026-08-29 12:52:52 | 7 | |
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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1 Resource Report Resource Website |
RRID:Addgene_118162 | KRAB-dCas9-2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | This plasmid is intended to be used as negative control for experiments using gRNAs cloned into Addgene Plasmid #118155 Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function. | Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:52 | 0 | |
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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3 Resource Report Resource Website |
RRID:Addgene_118164 | KRAB-dCas9-2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | This plasmid is intended to be used as negative control for experiments using gRNAs cloned into Addgene Plasmid #118155 Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function. | Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:52 | 0 | |
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p426 46Q GPD Resource Report Resource Website 1+ mentions |
RRID:Addgene_1182 | htt 46Q | Homo sapiens | Ampicillin | PMID:10677504 | N terminal region of Ht with polyQ repeat length of 46 fused to GFP. | Backbone Size:0; Vector Backbone:p426 GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:52 | 1 | |
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HsSPHK2 Resource Report Resource Website |
RRID:Addgene_118092 | Homo sapiens Sphingosine Kinase 2 | Homo sapiens | Ampicillin | PMID:29672528 | Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:51 | 0 | ||
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HsSPHK1 Resource Report Resource Website |
RRID:Addgene_118091 | Homo sapiens Sphingosine Kinase 1 | Homo sapiens | Ampicillin | PMID:29672528 | Backbone Size:5800; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:55 | 0 | ||
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pET23b-Casp2-Delta Card Resource Report Resource Website |
RRID:Addgene_11810 | Caspase-2 Delta Card | Homo sapiens | Ampicillin | Backbone Size:3666; Vector Backbone:pET-23 b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Delta Card | 2026-08-29 12:52:55 | 0 | ||
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lentiSAMv2 OPTN TSS-guide4 Resource Report Resource Website |
RRID:Addgene_118199 | dCas9-VP64-2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. | Backbone Marker:Feng Zhang (Addgene #75112); Vector Backbone:lentiSAMv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:56 | 0 | |
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lentiSAMv2 OPTN TSS-guide3 Resource Report Resource Website |
RRID:Addgene_118198 | dCas9-VP64-2A-BlastR | Homo sapiens | Ampicillin | PMID:31253982 | Please visit https://www.biorxiv.org/content/early/2018/08/27/400291 for bioRxiv preprint. | Backbone Marker:Feng Zhang (Addgene #75112); Vector Backbone:lentiSAMv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:52 | 0 | |
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pLenti-EB1-EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_118084 | human EB1 | Homo sapiens | Ampicillin | Please refer to pLenti-IFT20-EGFP for cloning info. | Vector Backbone:pEF1α-IRES-EGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:51 | 7 | ||
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pcDNA3-Casp7-Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_11815 | Caspase 7 | Homo sapiens | Ampicillin | PMID:9325297 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:51 | 6 | ||
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pcDNA3-Casp8 C360A Resource Report Resource Website 1+ mentions |
RRID:Addgene_11818 | Caspase 8 catalytic mutant | Homo sapiens | Ampicillin | PMID:9325297 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | C360A | 2026-08-29 12:52:55 | 5 | |
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pcDNA3-Casp8 Resource Report Resource Website 10+ mentions |
RRID:Addgene_11817 | Caspase 8 | Homo sapiens | Ampicillin | PMID:9325297 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:52 | 14 | ||
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pcDNA3-Casp2 C303A-Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_11812 | Caspase-2 catalytic mutant | Homo sapiens | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | C303A | 2026-08-29 12:52:51 | 2 | ||
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pcDNA3-Casp2-Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_11811 | Caspase-2 | Homo sapiens | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 12:52:51 | 8 | |||
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pcDNA3-Casp3 C163A-myc Resource Report Resource Website 1+ mentions |
RRID:Addgene_11814 | Caspase 3 catalytic mutant | Homo sapiens | Ampicillin | PMID:9325297 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | C163A | 2026-08-29 12:52:51 | 7 |
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