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Species: Homo sapiens
Genetic Insert: 3' UTR LEMD2-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117349 Copy
Species: Homo sapiens
Genetic Insert: SMAD1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8653785
Proper citation: RRID:Addgene_11735 Copy
Species: Homo sapiens
Genetic Insert: SMAD2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8752209
Proper citation: RRID:Addgene_11734 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR TP73-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117333 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR ASIC1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117339 Copy
Species: Synthetic
Genetic Insert: gRNA miR-124-1-3'
Vector Backbone Description: Backbone Marker:Zhang Lab; Backbone Size:9157; Vector Backbone:PX459; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: Cas9 with NLS included
Proper citation: RRID:Addgene_117324 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR CTDSP1-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117328 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-CTRL
Vector Backbone Description: Backbone Marker:Church Lab; Backbone Size:7460; Vector Backbone:PB-TRE; Vector Types:Mammalian Expression, Transposon-mediated integration; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' BamHI cloning site not destroyed, 3' BamHI cloning site destroyed
Proper citation: RRID:Addgene_117319 Copy
Species: E. coli
Genetic Insert: b-lactamase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11602576
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.
The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.
Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
Proper citation: RRID:Addgene_11724 Copy
Species: Mus musculus
Genetic Insert: Sox9
Vector Backbone Description: Backbone Size:9063; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30127505
Proper citation: RRID:Addgene_117269 Copy
Species: Mus musculus
Genetic Insert: Nfib
Vector Backbone Description: Backbone Size:10013; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30127505
Proper citation: RRID:Addgene_117271 Copy
Species: Cas9 (Streptococcus pyogenes M1 GAS)
Genetic Insert: Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
Vector Backbone Description: Backbone Marker:Sheng Yang, Addgene Plasmid #62225; Backbone Size:4476; Vector Backbone:pCas; Vector Types:other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30927506
Proper citation: RRID:Addgene_117259 Copy
Genetic Insert: SuperFolder GFP
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26883397
Proper citation: RRID:Addgene_117402 Copy
Species: Synthetic
Genetic Insert: SauCas9 sgRNA targeting TLR 2.0
Vector Backbone Description: Backbone Marker:Addgene#50920; Vector Backbone:pLKO.1-puro-U6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36070530
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117409 Copy
Genetic Insert: SuperFolder GFP
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26883397
Proper citation: RRID:Addgene_117404 Copy
Species: Synthetic
Genetic Insert: SpyCas9 sgRNA targeting TLR 2.0
Vector Backbone Description: Backbone Marker:Addgene#50920; Vector Backbone:pLKO.1-puro-U6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36070530
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117406 Copy
Species: Homo sapiens
Genetic Insert: 3' UTR TMEM134-201
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7334; Vector Backbone:pmiRGLO; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: 5' NheI cloning site destroyed, 3' XbaI cloning site destroyed
Proper citation: RRID:Addgene_117371 Copy
Species: Homo sapiens
Genetic Insert: PSD2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117463 Copy
Species: Homo sapiens
Genetic Insert: SMAD3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702197
Proper citation: RRID:Addgene_11742 Copy
Species: Homo sapiens
Genetic Insert: Cyth3
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117454 Copy
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